Biotinylation, the process of attaching biotin to a molecule, has become a ubiquitous technique in molecular biology and biotechnology. The biotin moiety's unparalleled affinity for streptavidin and avidin makes it an exceptionally useful tag for detection, purification, and immobilization of biomolecules. Among the diverse array of biotinylation reagents, Biotin-Maleimide (CAS: 139554-72-6) holds a prominent position due to its specific reactivity and versatility. NINGBO INNO PHARMCHEM CO.,LTD. is a key provider of such high-quality biotinylation reagents.

Biotinylation reagents differ in their reactive groups, targeting specific functional groups on biomolecules. Biotin-Maleimide is a prime example of a heterobifunctional crosslinker. It features a maleimide group, which is highly reactive towards sulfhydryl (thiol) groups commonly found in cysteine residues. This specificity is a major advantage, as it allows for targeted labeling of proteins, antibodies, and peptides without significant interference with other functional groups like amines or carboxyls. The resulting biotinylated molecule can then be easily detected or purified using streptavidin-conjugated systems.

In life science research, the applications of Biotin-Maleimide are extensive. It is widely used for labeling proteins to facilitate their study in various experimental setups. For instance, in protein-protein interaction studies, one protein can be biotinylated using Biotin-Maleimide, and then its binding partners can be isolated using streptavidin-coated beads followed by Western blot analysis. This technique provides a sensitive method for identifying molecular interactions.

Furthermore, Biotin-Maleimide is instrumental in creating tagged nucleic acids for molecular diagnostics and research. Biotinylated DNA or RNA probes can be used in hybridization assays, Southern blots, and Northern blots, where the biotin tag allows for sensitive detection via streptavidin-conjugated reporters. The ability to perform biomolecule labeling with Biotin-Maleimide ensures that these probes are functional and easily detectable.

The ease of use and reliability associated with streptavidin detection make Biotin-Maleimide a preferred choice for many researchers. The strength of the biotin-streptavidin interaction (Kd ≈ 10⁻¹⁴ M) ensures stable binding and signal amplification, even for low-concentration targets. When considering the purchase of Biotin-Maleimide, it's essential to source from reputable manufacturers like NINGBO INNO PHARMCHEM CO.,LTD., who guarantee product purity and consistent performance. Understanding the biotin maleimide chemical properties, including its typical solid form and storage requirements (-20°C, inert atmosphere), is key to successful implementation.

Moreover, Biotin-Maleimide is also valuable in the development of cell-based assays and imaging techniques. Cells can be labeled with biotinylated molecules to track their movement, study receptor-ligand interactions, or even for targeted delivery of imaging agents. The specificity of the maleimide reaction ensures that the labeling occurs at defined sites, contributing to clearer results.

In conclusion, Biotin-Maleimide is a powerful and versatile biotinylation reagent that plays a critical role in advancing life science research. Its ability to facilitate targeted labeling and its compatibility with sensitive detection systems make it an invaluable tool for a wide range of applications. NINGBO INNO PHARMCHEM CO.,LTD. is committed to empowering researchers by providing high-quality Biotin-Maleimide and supporting their groundbreaking discoveries.