Dabcyl acid, chemically known as 4-((4-(dimethylamino)phenyl)azo)benzoic acid, is a vital molecule in the field of fluorescence-based detection systems, particularly Förster Resonance Energy Transfer (FRET) assays. Its unique chemical properties make it an exceptional fluorescent quencher, enabling sensitive detection of molecular interactions and enzymatic activities.

The molecular formula of Dabcyl acid is C15H15N3O2, with a molecular weight of 269.3 g/mol. Its CAS registry number is 6268-49-1. Visually, Dabcyl acid typically appears as a yellow to amber to dark red powder or crystal. This characteristic color is indicative of its conjugated azo group, which is responsible for its broad absorption spectrum. The physical form and color are important considerations for researchers when sourcing this material, with many seeking to 'purchase Dabcyl acid' for their specific applications.

One of the key properties of Dabcyl acid is its strong absorption in the visible spectrum, particularly in the range that overlaps with many common donor fluorophores. This broad absorption band is crucial for its function as a quencher. When a donor fluorophore emits energy in close proximity to Dabcyl acid, the Dabcyl acid molecule absorbs this energy, thereby diminishing the fluorescence of the donor. This quenching efficiency is highly dependent on the spectral overlap and the distance between the donor and acceptor molecules.

Regarding its solubility, Dabcyl acid is known to be sparingly soluble in water but can be dissolved in organic solvents like DMSO (dimethyl sulfoxide) and DMF (dimethylformamide). For practical use in assays, it is often reconstituted in DMSO. Stability is another important factor; Dabcyl acid should be stored sealed in a dry environment at room temperature to maintain its integrity. Many suppliers of 'Dabcyl acid' provide detailed storage and handling instructions, often recommending protection from light as well.

The chemical reactivity of Dabcyl acid can be further modified through functionalization. For instance, Dabcyl acid succinimidyl ester (Dabcyl acid, SE) is a derivative that contains an amine-reactive succinimidyl ester group, allowing for covalent conjugation to proteins and peptides through their amino groups. This modification expands the utility of Dabcyl acid, enabling its incorporation into a wider range of biomolecules. When exploring options to 'buy Dabcyl acid', researchers may also consider these functionalized derivatives based on their specific conjugation needs.

Understanding the chemical properties and handling requirements of Dabcyl acid is essential for its effective use in FRET-based assays. Its role as a high-performance quencher, coupled with its adaptability through chemical modification, solidifies its importance in biochemical research and diagnostic tool development. The availability of high-purity Dabcyl acid from reliable 'Dabcyl acid manufacturers' ensures the quality and reproducibility of scientific experiments.