Fmoc-Leu-OH, scientifically known as Nα-Fmoc-L-leucine and identified by its CAS number 35661-60-0, is a highly significant molecule in the realm of organic chemistry and biochemistry. Its importance stems primarily from its role as a key building block in the synthesis of peptides, a process fundamental to drug discovery and biochemical research. Understanding its chemical properties is therefore crucial for researchers working with this compound.

Chemically, Fmoc-Leu-OH is characterized by its N-terminal Fmoc protecting group, which is labile under basic conditions. This feature is central to its utility in Fmoc solid-phase peptide synthesis (SPPS). The molecule's structure, C21H23NO4, with a molecular weight of 353.41, dictates its reactivity and solubility. It typically appears as a white to off-white solid and is soluble in solvents like DMSO, which is common for many organic reagents. The melting point, often cited around 152-156 °C, further characterizes its physical state.

The diverse applications of Fmoc-Leu-OH are a testament to its chemical versatility. In peptide synthesis, it is used to introduce the amino acid leucine into a growing peptide chain. This process is essential for creating peptides with specific biological functions, which are increasingly being explored as therapeutic agents. The chemical properties of Fmoc-Leu-OH ensure that the protection and deprotection steps in SPPS are efficient and controlled.

Beyond peptide synthesis, the compound finds utility in broader chemical synthesis projects. Researchers may use it as a chiral building block in the creation of complex organic molecules or as a starting material for synthesizing other valuable derivatives. The availability of high-quality amino acid derivatives for research, such as Fmoc-Leu-OH, is critical for the advancement of chemical sciences.

When sourcing this compound, it is vital to look for reliable suppliers who can guarantee its purity and provide comprehensive documentation, including Certificates of Analysis (CoA) and Material Safety Data Sheets (MSDS). The consistent quality of N-Fmoc-L-leucine is paramount for achieving accurate and reproducible results in any experimental setting. Companies specializing in peptide synthesis reagents often offer this compound, ensuring it meets the rigorous demands of modern research laboratories.

In summary, Fmoc-Leu-OH (CAS 35661-60-0) is a cornerstone reagent for anyone involved in peptide synthesis or advanced organic chemistry. Its well-defined chemical properties and broad range of applications make it an indispensable tool for scientific exploration and innovation.