Fmoc-L-phenylalanine (Fmoc-Phe-OH) is a staple reagent in peptide synthesis laboratories, recognized for its utility in introducing phenylalanine residues. Understanding its chemical properties, including solubility and stability, is crucial for its effective handling and optimal performance in research applications. Proper storage and careful handling practices ensure the integrity of this valuable amino acid derivative.

The molecular structure of Fmoc-Phe-OH imparts specific chemical characteristics. It is an N-protected amino acid, with the Fmoc group providing base-labile protection to the alpha-amino nitrogen. This allows for selective removal under mild basic conditions, a key advantage in multi-step synthesis. L-phenylalanine itself is an aromatic amino acid, contributing a hydrophobic character to the molecule. This hydrophobicity influences its solubility; Fmoc-Phe-OH is typically reported as insoluble in water but readily soluble in polar organic solvents like Dimethylformamide (DMF) and N-Methyl-2-pyrrolidone (NMP). This solubility profile is essential for its use in solid-phase peptide synthesis (SPPS), where it is dissolved and coupled to the growing peptide chain.

The purity of Fmoc-Phe-OH is a critical factor for research applications. High-purity material (often >99%) ensures efficient coupling reactions, minimizes side product formation, and leads to cleaner peptide products, reducing the need for extensive downstream purification. Researchers often rely on suppliers like NINGBO INNO PHARMCHEM CO.,LTD. who provide detailed Certificates of Analysis (CoA) that specify purity levels determined by techniques such as High-Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR).

Proper storage of Fmoc-Phe-OH is important to maintain its stability and purity. It is typically recommended to store the compound in a cool, dry place, often at temperatures around 4°C or even -20°C, depending on the specific supplier's recommendations and the intended duration of storage. Keeping the container tightly sealed helps to prevent moisture absorption and degradation.

When handling Fmoc-Phe-OH in a laboratory setting, standard laboratory safety practices should be followed. This includes wearing appropriate personal protective equipment (PPE) such as gloves, lab coats, and eye protection. While Fmoc-Phe-OH is not considered acutely toxic, avoiding inhalation of dust and skin contact is always advisable. Its CAS number, 35661-40-6, ensures accurate identification and documentation of the material used in experiments.

The cost of Fmoc-Phe-OH can vary, but researchers should prioritize obtaining it from reputable chemical suppliers who guarantee its quality and provide comprehensive handling and storage information. NINGBO INNO PHARMCHEM CO.,LTD. is one such supplier, offering high-quality Fmoc-Phe-OH that supports reliable and reproducible research outcomes. By understanding and adhering to best practices for handling and storing Fmoc-Phe-OH, researchers can maximize its effectiveness in their peptide synthesis endeavors.