Biochemical assays are indispensable tools for unraveling the complex mechanisms of biological systems. They allow scientists to measure enzyme activity, study reaction kinetics, and identify potential modulators of biological pathways. In this context, specific substrates are crucial for accurate and reliable assay results. Z-Tyr-ONp (CAS 3556-56-7), also known as N-Benzyloxycarbonyl-L-tyrosine 4-nitrophenyl ester, is a well-established substrate widely used in biochemical assays, particularly for enzymes that cleave ester bonds.

The enzymatic utility of Z-Tyr-ONp stems from its molecular design. The 4-nitrophenyl ester group is a good leaving group, making it susceptible to hydrolysis by esterase enzymes. When such an enzyme cleaves the ester bond, it releases 4-nitrophenol, which is a colored compound. The rate at which this colored product is formed is directly proportional to the enzyme's activity. This colorimetric readout simplifies the quantification of enzyme activity, making it a convenient method for high-throughput screening and kinetic studies. Researchers often seek to understand the Z-Tyr-ONp price as it can influence the feasibility of large-scale enzymatic studies.

Z-Tyr-ONp is particularly noted for its use in assays involving proteases that possess esterase activity, such as certain kallikreins and leukocyte elastases. By monitoring the release of 4-nitrophenol, researchers can determine the catalytic efficiency and specificity of these enzymes. This information is vital for understanding disease mechanisms, such as inflammation and blood coagulation, where these enzymes play significant roles. The precision required for such assays means that the quality of the CAS 3556-56-7 peptide synthesis grade material is critical, even when used as an enzyme substrate.

The availability of Z-Tyr-ONp from reliable suppliers is essential for the continuity of biochemical research. When laboratories decide to buy Z-Tyr-ONp online for assay purposes, they are investing in a tool that can provide critical insights into enzyme function. The study of these enzymes and their substrates, including various N-protected amino acid derivatives applications, contributes to the development of new diagnostics and targeted therapies. NINGBO INNO PHARMCHEM CO.,LTD. ensures the consistent supply of high-quality Z-Tyr-ONp, supporting the advancement of biochemical understanding and the discovery of novel enzyme inhibitors and activators.