Understanding the precise chemical identity and synthesis of key intermediates is crucial for their effective application in research and development. 4-[(2,4-Dimethoxyphenyl)(Fmoc-amino)methyl]phenoxyacetic acid, a vital component in modern peptide synthesis and drug delivery, is a prime example. NINGBO INNO PHARMCHEM CO.,LTD. provides this advanced chemical entity, and this discussion aims to illuminate its structural features, common synthetic routes, and analytical validation methods.

At its core, 4-[(2,4-Dimethoxyphenyl)(Fmoc-amino)methyl]phenoxyacetic acid is designed for solid-phase peptide synthesis (SPPS). Its molecular formula is C₃₂H₂₉NO₇, with a molecular weight of approximately 539.58 g/mol. The molecule features a central backbone that includes a phenoxyacetic acid group, which attaches to a resin support, and an amine functionality protected by the 9-fluorenylmethoxycarbonyl (Fmoc) group. This Fmoc group is essential for the iterative deprotection and coupling cycles characteristic of Fmoc-SPPS, offering UV detectability and selective removal under basic conditions.

The synthesis of this linker typically involves several key steps. One common approach begins with the functionalization of a phenoxyacetic acid derivative. This is then coupled with a suitably protected amine precursor derived from 2,4-dimethoxybenzaldehyde. The Fmoc protection is usually introduced onto the amino group at an appropriate stage. The overall process requires careful control of reaction conditions, including the choice of solvents, coupling agents (such as carbodiimides like DIC, often in conjunction with additives like HOBt), and reaction times to ensure high yield and purity. NINGBO INNO PHARMCHEM CO.,LTD. meticulously controls these parameters during manufacturing to deliver a consistent product.

Analytical techniques play a critical role in confirming the identity and purity of 4-[(2,4-Dimethoxyphenyl)(Fmoc-amino)methyl]phenoxyacetic acid. High-Performance Liquid Chromatography (HPLC) is routinely used to assess purity, typically achieving ≥98% for this compound. Nuclear Magnetic Resonance (NMR) spectroscopy, particularly ¹H NMR, provides detailed structural confirmation by identifying characteristic proton signals for the Fmoc aromatic protons, the phenoxy methylene protons, and the dimethoxyphenyl moiety. Mass spectrometry (MS), such as electrospray ionization mass spectrometry (ESI-MS), is used to verify the exact molecular weight, confirming the elemental composition.

The consistent quality of 4-[(2,4-Dimethoxyphenyl)(Fmoc-amino)methyl]phenoxyacetic acid, as supplied by NINGBO INNO PHARMCHEM CO.,LTD., is vital for researchers and manufacturers. Its reliable chemical identity and high purity ensure the success of complex peptide synthesis and the development of innovative drug delivery systems. Understanding its chemical underpinnings and synthesis pathways empowers users to leverage its full potential in advancing scientific discovery.