In the dynamic field of life sciences, precise and reliable detection methods are paramount. At NINGBO INNO PHARMCHEM CO.,LTD., we understand the critical need for high-quality reagents that drive scientific discovery. One such indispensable tool is the chromogenic substrate, and among the most vital is Disodium 5-bromo-4-chloro-1H-indol-3-yl Phosphate, often referred to by its acronym BCIP.

BCIP, with its CAS number 102185-33-1, is a cornerstone in various biochemical assays, primarily functioning as a substrate for alkaline phosphatase (AP). When AP acts upon BCIP, it cleaves the phosphate group, leading to the formation of an insoluble blue precipitate. This visual signal is the basis for its widespread use in colorimetric detection. The significance of this reaction cannot be overstated; it provides a clear, quantifiable readout, essential for interpreting the results of complex biological experiments.

One of the most prominent applications of BCIP is in conjunction with Nitro Blue Tetrazolium (NBT) in blotting techniques. The BCIP/NBT system generates a distinct purple-blue precipitate, offering superior sensitivity and resolution for detecting specific proteins or nucleic acids. This is particularly critical in Western blotting, where researchers aim to identify and quantify target proteins in complex biological samples. The ability to accurately visualize these targets is fundamental to understanding cellular pathways, disease mechanisms, and drug efficacy. Furthermore, the reliability of this system aids researchers in obtaining repeatable results, a hallmark of robust scientific practice.

Beyond blotting, BCIP is a crucial reagent for immunohistochemistry (IHC) and in situ hybridization (ISH). In IHC, it allows for the precise localization of antigens within tissue samples, enabling researchers to study protein expression patterns in their native cellular environment. Similarly, in ISH, BCIP helps visualize the location of specific nucleic acid sequences within cells or tissues, providing insights into gene expression and cellular function. The development of precise analytical techniques for molecular targets is often supported by the availability of high-quality chemical compounds.

The consistent performance of BCIP is a testament to the advancements in chemical synthesis and purification. At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to providing reagents that meet the highest standards of purity and efficacy. Our focus on delivering exceptional chemical products ensures that scientists can rely on our materials for critical experiments. Whether you are looking to buy BCIP for your research or are interested in exploring the broader spectrum of our biochemical intermediates, our team is dedicated to supporting your endeavors.

The demand for reliable reagents that facilitate advanced molecular biology techniques continues to grow. By understanding the intricate requirements of modern research, NINGBO INNO PHARMCHEM CO.,LTD. strives to be a leading supplier of essential biochemicals. We aim to empower scientific progress by ensuring that researchers have access to the tools they need to push the boundaries of knowledge. For those seeking to purchase high-quality biochemicals, exploring our product catalog is the first step towards achieving breakthrough results.