In the dynamic field of molecular biology, the precise isolation and manipulation of genetic material are paramount. Central to achieving these goals is the enzyme known as Proteinase K. From the initial stages of DNA and RNA extraction to complex protein analysis, Proteinase K plays an indispensable role, ensuring the purity and integrity of biological samples. Developed and supplied by NINGBO INNO PHARMCHEM CO.,LTD., this high-quality enzyme is a cornerstone for researchers worldwide.

Proteinase K, also recognized as protease K or endopeptidase K, is a serine protease characterized by its broad substrate specificity. Originally isolated from the fungus Engyodontium album, it possesses the remarkable ability to digest a wide array of proteins, including keratin, hence its name. This proteolytic power makes it exceptionally effective in removing contaminating proteins that are often present during the isolation of nucleic acids. Without the intervention of Proteinase K, these contaminating proteins can interfere with downstream applications, leading to inaccurate results.

One of the most critical functions of Proteinase K is its capacity to inactivate DNases and RNases. These enzymes, which degrade DNA and RNA respectively, are ubiquitous in biological samples. By adding Proteinase K to nucleic acid preparations, researchers effectively neutralize these degrading agents, thereby protecting the precious DNA and RNA from degradation. This protective action is vital for obtaining high-quality, intact nucleic acids, which are essential for subsequent analyses such as PCR, sequencing, and gene expression studies. The ability to purchase Proteinase K powder for molecular biology applications ensures that researchers have access to this crucial reagent.

The efficacy of Proteinase K is further enhanced by its stability across a wide range of conditions. It remains active in the presence of chemicals that denature proteins, such as Sodium Dodecyl Sulfate (SDS) and urea. This characteristic is particularly advantageous during cell lysis, where denaturants are often used to break open cells and release their contents. Furthermore, Proteinase K is not inhibited by chelating agents like EDTA, which are commonly used to sequester divalent cations and inhibit magnesium-dependent enzymes, including many nucleases. This resilience allows Proteinase K to function optimally even in complex reaction mixtures. For those looking to buy Proteinase K powder, the assurance of its robust performance in various buffer systems is a significant advantage.

The availability of high purity Proteinase K, often specified with an assay of 99%, signifies its suitability for demanding scientific applications. This exceptional purity minimizes the risk of introducing unwanted contaminants into experiments. Whether it's for general protein digestion or specialized nucleic acid purification, the reliability of medical grade Proteinase K powder is a testament to its quality. NINGBO INNO PHARMCHEM CO.,LTD. provides this essential enzyme, enabling scientists to achieve consistent and reproducible results in their research endeavors. Sourcing high purity Proteinase K CAS 39450-01-6 is a critical step for any laboratory focused on cutting-edge molecular biology.