In the dynamic field of molecular biology, precise tools are paramount for advancing scientific understanding and developing innovative solutions. Hygromycin B, a powerful antibiotic derived from Streptomyces hygroscopicus, stands out as an indispensable reagent for genetic selection and cell culture applications. NINGBO INNO PHARMCHEM CO.,LTD. is proud to be a trusted supplier of high-quality Hygromycin B, enabling researchers worldwide to achieve their scientific objectives.

The primary utility of Hygromycin B lies in its potent ability to inhibit protein synthesis. It achieves this by interfering with the 70S ribosomal translocation process and inducing misreading of the messenger RNA (mRNA) template. This fundamental mechanism not only allows it to combat bacterial, fungal, and even higher eukaryotic cells but also makes it an exceptional tool for genetic manipulation. Specifically, Hygromycin B acts as a selective marker, identifying and allowing the proliferation of cells that have been successfully engineered to contain the hygromycin resistance gene (hyg or hph). This gene encodes for hygromycin B phosphotransferase, an enzyme that inactivates Hygromycin B, conferring resistance to the antibiotic.

The application of Hygromycin B in establishing stable cell lines is particularly noteworthy. Researchers often utilize it to screen and maintain cells that have undergone successful transfection. The process typically involves exposing cells to Hygromycin B at specific concentrations, which vary depending on the cell type. Cells without the resistance gene are eliminated, while those that have incorporated the gene thrive. This precise selection process is fundamental for many experiments, including gene expression studies, protein production, and the development of cellular models for disease research. Understanding the appropriate Hygromycin B cell culture concentration is key to optimizing this selection process.

Beyond its role in genetic selection, Hygromycin B has also garnered attention for its potential as an antiviral agent. It has been observed that Hygromycin B can selectively penetrate cells that exhibit increased membrane permeability due to viral infection, thereby inhibiting viral translation. Furthermore, in agricultural applications, Hygromycin B has been explored for its insect-repellent properties when incorporated into animal feed, offering a dual benefit in livestock management.

As a leading supplier of specialty chemicals, NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing researchers with the high-purity compounds necessary for groundbreaking discoveries. Our commitment to quality ensures that each batch of Hygromycin B meets stringent standards, empowering your research into protein synthesis inhibition, genetic modification, and other critical life science applications. Whether you are investigating the Hygromycin B antibiotic mechanism or establishing a new selective cell line, trust us for your supply needs.