At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to supplying the global scientific community with high-quality chemical intermediates that enable groundbreaking research. Protein conjugation, the process of covalently attaching molecules to proteins, is a fundamental technique in biochemistry, diagnostics, and therapeutics. Fluorescent labeling of proteins is a particularly powerful application, allowing for sensitive detection, tracking, and quantification in various biological systems. Among the array of fluorescent dyes available, 5-Carboxytetramethylrhodamine stands out due to its exceptional purity and performance characteristics.

5-Carboxytetramethylrhodamine, identified by its CAS number 91809-66-4, is a purified single isomer of a rhodamine derivative. This high degree of purity is crucial for reproducible protein conjugation. When proteins are labeled, the efficiency and specificity of the labeling reaction can be influenced by the isomeric composition of the dye. Using a single-isomer dye like 5-carboxytetramethylrhodamine minimizes batch-to-batch variability and ensures that the spectral and reactive properties of the dye are consistent, leading to more reliable experimental outcomes. This is especially important in diagnostic applications where consistency is paramount for accurate results.

The applications for fluorescently labeled proteins are vast. They are integral to antibody production for immunofluorescence microscopy and flow cytometry, enabling the study of cellular structures and functions. In enzyme-linked immunosorbent assays (ELISAs) and other immunoassay formats, fluorescently labeled antibodies provide sensitive detection systems. Furthermore, labeled proteins are used in drug discovery to monitor drug-target interactions and in cell biology to track protein localization and movement within cells. The robust orange-red emission of 5-carboxytetramethylrhodamine makes it suitable for multiplexing with other fluorescent probes and for applications requiring strong signal detection.

For researchers looking to purchase 5-carboxytetramethylrhodamine, NINGBO INNO PHARMCHEM CO.,LTD. provides a reliable source for this critical intermediate. Our commitment to quality ensures that the products we deliver meet stringent specifications, supporting the critical needs of our clients in pharmaceutical development, academic research, and biotechnology. The availability of this dye in various packaging sizes allows researchers to scale their projects effectively, from initial feasibility studies to larger-scale production.

In summary, the strategic use of high-purity fluorescent dyes like 5-carboxytetramethylrhodamine significantly enhances the precision and reliability of protein conjugation techniques. NINGBO INNO PHARMCHEM CO.,LTD. is proud to support scientific advancement by providing access to such essential chemical building blocks, empowering researchers to achieve their most ambitious goals.