Bromophenol Blue (BPB) is a chemical compound that holds significant importance in scientific research laboratories due to its dual functionality as a pH indicator and a tracking dye. This article will delve into its key chemical properties and explore its wide range of applications, highlighting why it remains an essential reagent for many scientific disciplines. NINGBO INNO PHARMCHEM CO.,LTD. provides access to high-quality Bromophenol Blue for these critical research needs.

Chemically, Bromophenol Blue is known by its IUPAC name, 3,3-Bis(3,5-dibromo-4-hydroxyphenyl)-2,1λ6-benzoxathiole-1,1-dione, and its molecular formula is C19H10Br4O5S. Its CAS number is 115-39-9. One of its most notable properties is its behavior as an acid-base indicator. The compound exhibits a distinct color change within the pH range of 3.0 to 4.6. In acidic environments (pH < 3.0), it appears yellow, and as the solution becomes more alkaline (pH > 4.6), it turns blue. This reversible color transition is due to changes in its molecular structure and protonation state, making it a reliable indicator for determining acidity or alkalinity.

Beyond its use in pH determination, Bromophenol Blue is extensively used as a tracking dye in gel electrophoresis. Its negative charge at neutral pH allows it to migrate in the same direction as DNA and proteins. This visual cue is invaluable for monitoring the progress of electrophoresis runs. Researchers can easily observe the migration front of the dye, helping them to control the separation process and prevent samples from running off the gel. The availability to buy Bromophenol Blue online ensures that researchers have access to this vital tool for their molecular separation experiments.

The solubility profile of Bromophenol Blue also contributes to its ease of use. It is soluble in water and common organic solvents such as ethanol and methanol. This allows for the preparation of solutions at desired concentrations for various applications. Whether it's for a simple titration or a more complex biochemical assay, understanding the uses of Bromophenol Blue is key to its effective implementation. The cost-effectiveness of this reagent further cements its place in laboratories worldwide.

In biochemical studies, Bromophenol Blue can also be used in certain assays where a visual indication of reaction progress or molecular binding is required. Its ability to bind to proteins, for example, can be utilized in specific detection methods. The consistent quality of Bromophenol Blue, procurable from reputable suppliers like NINGBO INNO PHARMCHEM CO.,LTD., is essential for reproducible and accurate scientific results. The chemical properties of this compound make it a versatile workhorse in any research laboratory.