In the realm of scientific research, precise measurement and clear observation are paramount. Among the array of essential laboratory chemicals, Bromophenol Blue stands out as a remarkably versatile compound. This article delves into the core aspects of Bromophenol Blue, particularly its critical role as a pH indicator and its utility in the field of electrophoresis.

Bromophenol Blue, identified by its CAS number 115-39-9, is a sulfonephthalein dye. Its primary claim to fame lies in its ability to undergo a distinct color change within a specific pH range. Typically, Bromophenol Blue appears yellow in acidic solutions, specifically below a pH of 3.0. As the pH increases, it transitions through shades of green and eventually turns blue at or above a pH of 4.6. This predictable color shift makes it an invaluable tool for researchers needing to monitor or indicate the pH of solutions during experiments, titrations, or other analytical procedures. The reliability of the bromophenol blue pH indicator range is a key factor in its widespread adoption.

Beyond its function as a pH indicator, Bromophenol Blue is widely employed as a tracking dye in gel electrophoresis. In techniques like agarose or polyacrylamide gel electrophoresis, where DNA, RNA, or proteins are separated based on size and charge, a visual marker is needed to follow the progress of the separation. Bromophenol Blue serves this purpose effectively. Migrating through the gel alongside the sample, its presence allows researchers to estimate the progress of the electrophoresis run and determine when to stop it, ensuring optimal separation. The ability to buy Bromophenol Blue online from various suppliers ensures its accessibility for these crucial applications.

The chemical properties of Bromophenol Blue further enhance its utility. It is generally soluble in water and common organic solvents like ethanol, allowing for flexibility in preparation and application. The stability of Bromophenol Blue under typical laboratory conditions makes it a dependable reagent for consistent results. Many scientific research endeavors rely on the consistent performance of such fundamental chemicals. For those seeking to purchase this essential reagent, understanding where to find high-quality Bromophenol Blue is key to successful experiments.

The cost-effectiveness and ease of use of Bromophenol Blue contribute significantly to its popularity. Whether for routine pH checks or for monitoring complex electrophoretic separations, it provides clear, actionable visual feedback. NINGBO INNO PHARMCHEM CO.,LTD. recognizes the importance of such fundamental chemicals and aims to provide reliable access to them for the scientific community. Understanding the uses of Bromophenol Blue in the laboratory is fundamental for students and seasoned researchers alike, solidifying its position as an indispensable laboratory chemical.