The effectiveness of gel electrophoresis, a cornerstone technique in molecular biology, is profoundly influenced by the specifications of the agarose used. Understanding these parameters is crucial for researchers to select the most appropriate agarose for their specific applications, whether it's routine DNA electrophoresis, sensitive blotting, or protein analysis. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality Agarose Powder with clearly defined specifications to guide your selection.

One of the most critical specifications for agarose is its Electroendosmosis (EEO) value. EEO refers to the net negative charge of the agarose polymer, which can affect the migration of nucleic acids. A high EEO can lead to a distorted electrophoretic front and reduced resolution. Our Agarose Powder is characterized by a low EEO (≤ 0.13), which minimizes this effect, ensuring cleaner band separation and improved resolution, especially for DNA fragments. This low EEO is a significant advantage for anyone looking to buy agarose for precise DNA electrophoresis.

Gel strength is another vital specification, indicating the rigidity of the agarose gel at a given concentration. Measured typically at 1% concentration, a higher gel strength (e.g., ≥1200 g/cm²) implies that the gel is robust and less prone to breakage. This is particularly important during handling, transfer for blotting, or when working with low agarose concentrations. The strong gel matrix provided by our product enhances ease of use and reduces the risk of experimental errors, making it a reliable choice for molecular biology applications where sample integrity is paramount.

The melting and gelling points of agarose are also important considerations. Gelling point refers to the temperature at which the agarose solution solidifies, while melting point is the temperature at which the gel liquefies. Our Agarose Powder has a gelling point of 36°C ±1.5°C and a melting point of 88°C ±1.5°C (for 1.5% gels). These standard temperatures are suitable for most routine applications, including DNA/RNA electrophoresis and various blotting techniques. For specialized applications, such as recovery of DNA from gels for downstream processing, low melting point agaroses are available, but for general research, our product offers a balanced performance.

The purity of the agarose, as indicated by parameters like sulfate content and ash content, also plays a role in its performance. Low levels of these impurities contribute to clearer gels and better resolution. Coupled with the assurance of being free from DNase, RNase, and protease, these specifications underscore the quality of NINGBO INNO PHARMCHEM CO.,LTD.'s molecular biology grade agarose. Researchers can confidently purchase our agarose powder for a wide range of molecular biology experiments, secure in the knowledge that they are using a reagent designed for optimal performance in nucleic acid separation.

By understanding these key specifications, scientists can make informed decisions when selecting agarose, ensuring the success of their research endeavors, from basic gel electrophoresis to more complex molecular analyses.