In the realm of analytical chemistry, precision and sensitivity are paramount. O-Phthalaldehyde (OPA) has carved a significant niche as a reagent that enhances these very qualities, primarily through its ability to participate in fluorometric detection methods. Its reactivity with primary amines, coupled with the fluorescent nature of the resulting products, makes it an exceptionally useful tool for analyzing a wide array of substances.

The principal application of OPA in chemical analysis lies in its derivatization of primary amines, amino acids, peptides, and proteins. When OPA reacts with a primary amine in the presence of a thiol, it forms a highly fluorescent isoindole derivative. This reaction is rapid and can be performed under mild conditions, often at room temperature, making it convenient for many analytical workflows. The resulting fluorescence is typically measured at specific excitation and emission wavelengths, allowing for sensitive quantification even at very low concentrations (nanogram to picomole levels).

This fluorometric derivatization is widely employed in various analytical techniques. For instance, in High-Performance Liquid Chromatography (HPLC), OPA is used as a pre-column or post-column derivatization reagent to detect and quantify amino acids in complex matrices like food samples, biological fluids, and environmental samples. Its sensitivity is particularly beneficial when analyzing samples with low analyte concentrations or when high specificity is required.

Beyond amino acids, OPA is also utilized for the analysis of other biologically relevant molecules. It has been used for the determination of diamines, certain amino alcohols, and even for quantifying proteins by reacting with their terminal amino groups. The versatility of OPA as a chemical reagent makes it a go-to choice for laboratories engaged in detailed chemical profiling and quality control.

NINGBO INNO PHARMCHEM CO.,LTD. offers high-quality O-Phthalaldehyde to support your advanced analytical needs. Our commitment to purity ensures that you receive a reagent that delivers consistent and reliable performance in your fluorometric assays. For any laboratory or research institution requiring precise amino acid detection or sensitive quantification of primary amines, our OPA is an ideal solution. Contact us to learn more about our competitive prices and bulk purchase options.