Biochemical assays are the bedrock of much biological and medical research, providing quantitative data on enzyme activity, metabolite concentrations, and molecular interactions. The development of sensitive and specific assay substrates is paramount to the success of these investigations. Chromogenic substrates, which produce a colored product upon reaction with a specific enzyme, are particularly valuable due to their ease of detection and quantification via spectrophotometry.

Among these vital tools is H-D-Ala-pNA HCl (CAS: 201731-77-3), a compound expertly designed as a chromogenic substrate for D-aminopeptidases. Its molecular structure is ingeniously crafted: the D-alanine moiety serves as the recognition site for the target enzyme, while the p-nitroanilide (pNA) group acts as the chromophore. When a D-aminopeptidase enzyme encounters and cleaves H-D-Ala-pNA HCl, it breaks the peptide bond, releasing free 4-nitroaniline. This liberated molecule is responsible for the characteristic yellow coloration, the intensity of which is directly proportional to the amount of enzyme activity present.

The application of H-D-Ala-pNA HCl in biochemical assays is broad. It is extensively used to:

  • Quantify D-aminopeptidase activity: This is its primary function, allowing researchers to measure the catalytic efficiency of these enzymes.
  • Screen for enzyme inhibitors: Researchers can test potential drug candidates or compounds to see if they inhibit D-aminopeptidase activity, indicated by a reduced color development.
  • Characterize novel D-aminopeptidases: When new D-aminopeptidases are discovered, H-D-Ala-pNA HCl can be used to determine their optimal conditions and substrate specificity.
  • High-throughput screening (HTS): Its colorimetric nature makes it suitable for automated HTS platforms, enabling the rapid screening of large compound libraries for enzyme modulators.

The precision and reliability offered by chromogenic substrates like H-D-Ala-pNA HCl are critical for reproducible scientific results. The ability to generate a clear, measurable color signal simplifies experimental setup and data analysis, making complex enzyme studies more accessible.

NINGBO INNO PHARMCHEM CO.,LTD. is proud to supply high-quality H-D-Ala-pNA HCl, ensuring that researchers have access to a dependable reagent for their biochemical assays. Our commitment to purity and consistency guarantees that your experiments will yield accurate and meaningful data. Trust NINGBO INNO PHARMCHEM CO.,LTD. to be your partner in advancing scientific understanding through reliable biochemical tools.

By providing essential chromogenic substrates like H-D-Ala-pNA HCl, NINGBO INNO PHARMCHEM CO.,LTD. empowers the scientific community to make critical discoveries in enzyme function and molecular biology.