NINGBO INNO PHARMCHEM CO.,LTD. is pleased to spotlight the crucial role of Nitrotetrazolium Blue Chloride (CAS 298-83-9) in the fields of Immunohistochemistry (IHC) and Immunocytochemistry (ICC). As a sensitive chromogenic substrate, Nitrotetrazolium Blue Chloride is instrumental in visualizing enzyme activity within cellular and tissue samples, enabling researchers to pinpoint specific biological processes with remarkable clarity.

In IHC and ICC protocols, Nitrotetrazolium Blue Chloride serves as a key detection reagent. When an antibody targets a specific protein or cellular component, an enzyme conjugated to that antibody can then catalyze the reduction of Nitrotetrazolium Blue Chloride. This reaction results in the formation of an insoluble, vividly colored precipitate—typically a deep blue—at the site of enzyme activity. This visual cue allows for the precise localization and assessment of the target molecule's presence and activity within its native cellular environment.

The compound's reliability in producing these distinct blue deposits makes it ideal for detecting a range of enzymatic activities, including those of dehydrogenases and oxidases. This capability is essential for studying cellular respiration, oxidative stress, and other metabolic functions. Moreover, its use in conjunction with BCIP for identifying superalkaline phosphatase activity further broadens its applicability in diagnostic and research settings. The accurate IHC and ICC staining achieved with this reagent supports detailed histological and cytological analyses.

NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing researchers with the high-quality reagents necessary for successful visualization techniques. Our Nitrotetrazolium Blue Chloride is manufactured to meet exacting standards, ensuring consistent performance in sensitive applications like IHC and ICC. By utilizing this vital enzyme substrate, scientists can gain deeper insights into cellular mechanisms and disease pathology, paving the way for future scientific advancements.