9-Fluorenylmethyl Chloroformate (FMOC-Cl), identified by CAS 28920-43-6, is a key organic compound with a well-defined set of chemical properties that underpin its significant utility across various scientific and industrial sectors. NINGBO INNO PHARMCHEM CO.,LTD. offers this essential chemical intermediate, enabling advancements in research and development.

Chemically, FMOC-Cl is characterized by its chloroformate ester functional group attached to the 9-fluorenylmethyloxy moiety. This structure makes it a reactive electrophile, particularly susceptible to nucleophilic attack by amines. The FMOC group's inherent UV absorption at approximately 265 and 300 nm (λmax=265/300 nm) is a critical feature that facilitates its monitoring and quantification in analytical processes. Its reactivity with water, leading to hydrolysis and the release of carbon dioxide, necessitates storage under controlled conditions, typically at 2-8°C under an inert atmosphere, to maintain its integrity. Its solubility in polar aprotic solvents like DMF further enhances its utility in various reaction media.

The primary application of FMOC-Cl is as a reagent for introducing the FMOC protecting group, most notably in peptide synthesis and amino acid analysis. In solid phase peptide synthesis, it reacts with the free amine of an amino acid attached to a resin, forming an FMOC-protected amino acid. This group is stable to acid but readily cleaved by a weak base like piperidine. This specific reactivity profile allows for selective deprotection and stepwise elongation of the peptide chain.

Beyond peptide chemistry, FMOC-Cl finds use in other areas of organic synthesis. It can be employed in the derivatization of amines for chromatographic analysis, enhancing detection sensitivity and improving separation characteristics. Its use as a building block for more complex molecules or as a reagent in the synthesis of specialty chemicals further illustrates its versatility. NINGBO INNO PHARMCHEM CO.,LTD. ensures that our FMOC-Cl possesses the required purity and stability for these diverse applications, supporting innovation in chemical research and industry.