NINGBO INNO PHARMCHEM CO.,LTD. is proud to be a leading supplier of specialized chemical compounds that drive innovation in biological research. Among our extensive catalog, Nitrotetrazolium Blue Chloride (CAS 298-83-9) holds a significant position due to its multifaceted applications as an enzyme substrate. This high-purity compound is instrumental in achieving precise and quantifiable results in a variety of biochemical and cellular analysis techniques. We are committed to being your trusted manufacturer in China for these critical materials.

As a substrate for dehydrogenases and peroxidases, Nitrotetrazolium Blue Chloride is frequently employed in the quantitative determination of enzyme activities. A prime example is its use in measuring glucose-6-phosphate dehydrogenase levels. This enzyme is a key indicator in various metabolic pathways, and accurate measurement is crucial for research in areas like cancer biology and red blood cell disorders. By offering a consistent and high-grade Nitrotetrazolium Blue Chloride, NINGBO INNO PHARMCHEM CO.,LTD. supports researchers in their pursuit of detailed scientific understanding. When you buy from us, you're assured of a product that delivers reliable performance, every time.

The compound's reaction with alkaline phosphatase to produce a vividly colored, insoluble formazan product makes it an invaluable component in immunohistochemistry (IHC) and immunocytochemistry (ICC). These techniques are foundational for visualizing the localization of specific proteins within tissue sections and cells, respectively. The blue precipitate formed by Nitrotetrazolium Blue Chloride acts as a clear signal, allowing scientists to pinpoint where a particular enzyme activity, or a downstream detection system, is active. This capability is crucial for understanding cellular processes and disease mechanisms.

In the realm of protein analysis, western blotting (WB) also benefits significantly from the use of Nitrotetrazolium Blue Chloride. Following the separation and transfer of proteins, enzyme-conjugated antibodies are used to detect specific target proteins. When Nitrotetrazolium Blue Chloride is added as a substrate, it reacts with the enzyme to produce a visible signal, allowing for the detection and semi-quantification of the protein of interest. This method is a cornerstone of molecular biology research, and the quality of the substrate directly impacts the clarity and reliability of the results.

Ningbo INNO PHARMCHEM CO.,LTD., as a dedicated manufacturer in China, ensures that our Nitrotetrazolium Blue Chloride meets the rigorous demands of scientific research. We offer competitive pricing for this essential chemical, making advanced research more accessible. We invite you to explore our product offerings and discover how our commitment to quality can benefit your laboratory's critical enzyme assays and protein analysis needs.