Accurate amino acid profiling is fundamental to numerous scientific disciplines, from clinical diagnostics to nutritional science and food analysis. At the heart of many advanced amino acid analysis techniques lies O-Phthalaldehyde (OPA), a reagent that transforms non-fluorescent amino acids into highly fluorescent derivatives, enabling their sensitive detection via HPLC. The efficacy of this process hinges significantly on the purity of the OPA used.

When scientists buy O-Phthalaldehyde online for amino acid analysis, the primary concern is often the reagent's purity. Even minute levels of impurities can lead to baseline noise, spurious peaks, or quenching of the fluorescent signal, all of which can distort the amino acid profile and lead to incorrect conclusions. Therefore, sourcing OPA with a guaranteed high purity, such as the 99% offered by Ningbo Inno Pharmchem Co., Ltd., is not merely a preference but a necessity for reliable quantitative results. This commitment to purity ensures that the fluorescent derivatization reaction proceeds cleanly, yielding accurate and reproducible data.

The process of O-Phthalaldehyde HPLC derivatization involves reacting OPA with primary amines, typically in the presence of a thiol, under alkaline conditions. The resulting isoindole compounds exhibit strong fluorescence, allowing for detection at picomolar levels. This sensitivity is critical when analyzing biological samples that may contain amino acids at very low concentrations. By utilizing a high-quality OPA reagent for peptides and amino acids, researchers can confidently identify and quantify each component, contributing to a deeper understanding of biological processes.

Beyond its analytical applications, the consistent quality of OPA is also valued in its use as a chemical intermediate and disinfectant. However, for analytical chemists, the paramount importance of purity in OPA remains undisputed. Ningbo Inno Pharmchem Co., Ltd. understands these critical requirements and is dedicated to supplying O-Phthalaldehyde that meets the highest standards, empowering scientific innovation through dependable analytical tools.