In the intricate world of molecular biology, precise separation of proteins is paramount for accurate analysis and research. Tricine, a zwitterionic buffer with the CAS number 5704-04-1, has emerged as a cornerstone for achieving high-resolution protein separations, particularly within Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) systems. Its unique chemical properties allow for the effective resolution of small to medium-sized proteins and peptides, which often prove challenging with traditional glycine-based buffers.

Tricine, derived from Tris and glycine, offers a distinct buffering range between pH 7.4 and 8.8. This characteristic makes it an ideal choice for electrophoretic systems where maintaining a stable pH is critical for the migration and separation of biomolecules. The development of Tricine-SDS-PAGE by Schägger and von Jagow revolutionized the separation of proteins in the 1 to 100 kDa range. This advancement is invaluable for researchers studying small proteins, peptides, and protein fragments, enabling clearer visualization and quantification of these often-elusive biological components.

The effectiveness of Tricine as a buffer lies in its ability to provide a stable ionic environment. Unlike glycine, Tricine exhibits a higher negative charge at typical running pH values, which influences its migration rate and interactions within the gel matrix. This tailored migration characteristic contributes to sharper band resolution and reduced diffusion, ultimately leading to more reliable experimental results. For scientists aiming to understand protein function, Purity Degree 99% Tricine CAS No. 5704-04-1 is a crucial parameter, ensuring consistency and accuracy in their findings.

Beyond its primary role in SDS-PAGE, Tricine finds utility in other biochemical applications. Its buffering capacity makes it suitable for sample preparation for mass spectrometry, where maintaining sample integrity is essential. Furthermore, it can be employed in nucleic acid extraction protocols, safeguarding delicate genetic material. The versatility of Tricine as a research chemical underscores its importance in modern biological laboratories. When seeking to buy Tricine, understanding its specific applications and purity levels is key to selecting the right grade for your research needs.

NINGBO INNO PHARMCHEM CO.,LTD. recognizes the critical role of high-quality biochemicals in scientific advancement. We are committed to supplying researchers with premium grade Tricine, ensuring that your experiments benefit from the precision and reliability that this essential buffer provides. Whether you are performing routine electrophoresis or exploring novel protein interactions, our Tricine will support your efforts to achieve unparalleled results.