In the realm of biochemical and immunological research, precision and sensitivity are paramount. Enzyme-linked immunosorbent assays (ELISA) have become a cornerstone technique, relied upon for everything from diagnostics to drug discovery. At the heart of a successful ELISA lies the choice of substrate, and ABTS diammonium salt stands out as a highly effective and versatile option. NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing high-quality reagents that empower researchers, and our ABTS diammonium salt is a testament to this commitment.

ABTS, or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, is a chromogenic substrate specifically designed for peroxidase-based enzymatic reactions. When combined with hydrogen peroxide and a peroxidase enzyme, ABTS undergoes oxidation to form a stable, intensely colored product – a distinct green hue. This reaction is the basis for quantitative detection in many immunoassay formats, including ELISA. The soluble nature of the resulting product allows for straightforward spectrophotometric measurement, typically at an absorbance maximum around 405 nm, offering a clear and reliable signal.

One of the key advantages of using ABTS diammonium salt is its sensitivity. Researchers can detect even minute quantities of the target analyte, making it ideal for applications where low expression levels or trace amounts are critical. This heightened sensitivity contributes to improved lower limits of detection (LLOD) in ELISA assays, leading to more robust and informative results. For those focusing on food science, the substrate is invaluable for measuring antioxidant capacities, a crucial parameter in assessing the quality and health benefits of food products. Understanding the antioxidant potential can inform product development and consumer choices.

Furthermore, the kinetics of enzyme reactions can be effectively monitored using ABTS diammonium salt. This is vital for studying enzyme activity, optimizing reaction conditions, and understanding biochemical pathways. Beyond research, its utility extends to clinical diagnostics, where it can be integrated into glucose estimating reagents for blood serum analysis, contributing to the accurate assessment of metabolic health. NINGBO INNO PHARMCHEM CO.,LTD. ensures that our ABTS diammonium salt meets stringent purity standards, guaranteeing consistent performance and reproducibility across experiments. When considering the buy or purchase of such critical reagents, ensuring quality and reliability is paramount, and our company stands ready to meet these demands.