Understanding the fundamental properties of the reagents we use is crucial for optimizing experimental design and achieving reliable results. MES Monohydrate, identified by CAS number 145224-94-8, is a widely utilized biological buffer whose efficacy stems directly from its unique chemical structure and properties. NINGBO INNO PHARMCHEM CO.,LTD. emphasizes the importance of this chemical understanding, providing researchers with reagents that not only perform but also possess well-defined characteristics for predictable outcomes.

At its core, MES Monohydrate is an N-substituted aminosulfonic acid, falling into the category of Good's buffers. Its zwitterionic nature is key to its buffering action. This means that at physiological pH, the molecule carries both a positive and a negative charge. Specifically, the morpholino group is protonated (positive charge), while the sulfonic acid group is deprotonated (negative charge). This internal charge balance contributes to its low reactivity and minimal interaction with biological molecules, making it an excellent buffer for sensitive biological systems. The pKa of MES Monohydrate is approximately 6.1 at 20°C, which makes it ideal for buffering in the pH range of 5.5 to 6.7. This particular pH range is optimal for many biochemical reactions and cellular processes.

The solubility of MES Monohydrate in water is also a significant advantage. It dissolves readily, allowing for the preparation of stock solutions of various concentrations. This is particularly useful for applications requiring precise buffering, such as in the preparation of cell culture media or enzyme assay buffers. The fact that it is generally transparent in UV light further enhances its utility in spectrophotometric analyses, as it does not significantly absorb light in the wavelengths commonly used to measure biomolecules. Researchers often look for long_tail_keyword such as 'MES Monohydrate solubility' to understand its practical handling.

NINGBO INNO PHARMCHEM CO.,LTD. ensures that their MES Monohydrate meets high purity standards, typically exceeding 99%. This level of purity is essential to prevent any interference with sensitive biological experiments. For instance, in protein purification using chromatography, impurities in the buffer could lead to non-specific binding or altered elution profiles, compromising the purity of the target protein. By offering high-purity MES Monohydrate, NINGBO INNO PHARMCHEM CO.,LTD. supports researchers in achieving accurate and reproducible results. As a reliable supplier, they understand the critical need for consistent quality.

The versatility of MES Monohydrate is evident in its application across different fields. From maintaining stable pH in enzyme assays and cell cultures to serving as a running buffer in electrophoresis, its chemical properties make it a valuable asset in any laboratory. When you consider the best way to buy such a vital reagent, seeking out a reputable manufacturer like NINGBO INNO PHARMCHEM CO.,LTD. is paramount. Their dedication to quality assurance means you can trust the product for your critical research needs.

In conclusion, the chemical properties of MES Monohydrate (CAS 145224-94-8) – its zwitterionic nature, moderate pKa, high solubility, and purity – make it an exceptionally useful buffer for a broad spectrum of biological research. Understanding these characteristics allows scientists to leverage its capabilities effectively. NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers with the highest quality MES Monohydrate, supporting scientific advancement through reliable chemical supply. Consider NINGBO INNO PHARMCHEM CO.,LTD. for your next purchase and experience the difference quality makes.