NINGBO INNO PHARMCHEM CO.,LTD. proudly offers Tetramethylbenzidine (TMB), a critical reagent in modern biochemical analysis and diagnostics. As a leading supplier of specialty chemicals, we emphasize the significance of TMB as a safer, highly effective chromogenic substrate compared to its predecessors.

The historical reliance on benzidine and its derivatives as chromogenic substrates for enzymes like Horseradish Peroxidase (HRP) posed significant health risks due to their carcinogenic nature. This led to a global push for safer alternatives. Tetramethylbenzidine, identified by its CAS number 54827-17-7, emerged as a leading candidate. Its molecular structure, featuring methyl substitutions that block carcinogenic activation pathways, makes it a non-mutagenic and non-carcinogenic option. This crucial difference ensures a safer working environment for laboratory personnel and reduces potential risks associated with handling. When considering the purchase of laboratory chemicals, this safety aspect is a primary driver for choosing TMB.

The utility of TMB extends to its excellent performance in enzyme-linked immunosorbent assays (ELISA) and other enzyme immunoassays (EIA). In these applications, HRP is typically conjugated to antibodies. Upon binding to the target analyte, the HRP is available to catalyze the oxidation of TMB. This enzymatic reaction produces a distinct color change, allowing for quantitative detection of the target substance. The sensitivity of TMB substrate for ELISA is notably high, enabling the detection of analytes at very low concentrations, which is vital for early disease diagnosis and precise scientific research.

The chemical properties of TMB also contribute to its popularity. The oxidation of TMB by HRP, in the presence of hydrogen peroxide, proceeds through intermediate stages that can produce different colors, primarily blue-green and then yellow. This versatility allows for different assay designs and read-out times. Furthermore, the acid-stop procedure, commonly employed in ELISA protocols, converts the blue-green intermediate to a stable yellow product while simultaneously amplifying the signal. This characteristic enhances the overall sensitivity and robustness of the assay.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing Tetramethylbenzidine of the highest purity, ensuring consistent performance for all its applications. Whether for diagnostic kit manufacturing, academic research, or clinical testing, reliable biochemical reagents are essential. Our Tetramethylbenzidine serves as a testament to our dedication to quality and safety, offering a scientifically sound and ethically superior choice for a wide range of applications. By choosing NINGBO INNO PHARMCHEM CO.,LTD., you are investing in a product that prioritizes both performance and well-being.