In the dynamic field of biochemistry, the ability to accurately detect and quantify biomolecules is paramount. O-Phthalaldehyde, commonly known as OPA, has emerged as an indispensable reagent, particularly for the analysis of amino acids. This powerful compound is celebrated for its capacity to react with primary amines, including those found in amino acids, to produce highly fluorescent derivatives. This reaction forms the cornerstone of many sensitive analytical techniques.

The process typically involves reacting OPA with the target amino acids in the presence of a thiol, such as 3-mercaptopropionic acid or N-acetyl-L-cysteine. This reaction, which can occur rapidly at room temperature, yields intensely fluorescent isoindole products. These fluorescent signals can then be readily detected and quantified using spectrofluorometry or chromatographic methods like High-Performance Liquid Chromatography (HPLC). The high sensitivity achieved with OPA-based assays allows for the detection of even picomole levels of amino acids, making it invaluable for analyzing biological samples such as plasma, tissue extracts, and cellular fluids.

The significance of OPA in biochemical analysis extends to its use in clinical diagnostics. For instance, it is employed in tests to measure urea nitrogen levels, which is critical for diagnosing and monitoring kidney and metabolic diseases. Furthermore, OPA's utility is recognized in protein quantification, where its reaction with the epsilon-amino group of lysine residues and the N-terminal amino groups of proteins allows for sensitive and rapid protein assays.

For researchers and laboratories seeking reliable and sensitive analytical tools, understanding the optimal conditions for OPA reactions is key. Factors such as pH, temperature, and the choice of thiol can influence the yield and stability of the fluorescent products. Despite its sensitivity, the product of the reaction is not always stable, necessitating immediate detection after derivatization.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing high-quality chemical reagents that drive scientific discovery. Our O-Phthalaldehyde is synthesized to meet stringent purity standards, ensuring reliable and reproducible results for your analytical needs. Explore how our O-Phthalaldehyde can enhance your amino acid detection assays and support your critical research objectives. We offer competitive prices for bulk purchases, making advanced biochemical analysis accessible.