Bicine (CAS 150-25-4) is a chemical compound that has gained significant traction in scientific research due to its excellent buffering capabilities. As a member of the Good's Buffers family, it is engineered to provide stable pH environments, typically ranging from 7.6 to 9.0, which are critical for numerous biological and biochemical processes. Understanding the specific Bicine uses in biochemistry is key to appreciating its value in the laboratory.

One of the most prominent applications of Bicine is in the field of protein crystallization. The process of forming protein crystals for structural analysis is notoriously sensitive to environmental conditions, with pH being a primary factor. Bicine's ability to maintain a consistent pH is instrumental in facilitating the ordered assembly of protein molecules into crystals. Researchers often seek to buy Bicine buffer specifically for this purpose, recognizing its contribution to achieving high-quality crystal growth.

In the realm of protein purification, Bicine is extensively utilized in chromatography, particularly ion exchange chromatography. This separation technique relies on the charge properties of proteins, which are directly influenced by the surrounding pH. Bicine ensures a stable pH throughout the chromatographic process, enabling the precise binding and elution of target proteins based on their charge characteristics. This makes Bicine a crucial reagent for obtaining pure protein samples for further study.

Beyond these applications, Bicine also finds utility in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), a fundamental technique for separating proteins based on their molecular weight. While not always the primary buffer, Bicine can be incorporated into buffer systems to help maintain the necessary pH for efficient protein migration through the gel matrix. Its consistent performance, even in complex buffer formulations, highlights its reliability.

The efficacy of Bicine in these diverse applications stems from its chemical properties, including its zwitterionic nature and limited interaction with metal ions. These characteristics contribute to its stability and prevent unintended side reactions that could compromise experimental results. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality Bicine, ensuring that researchers have access to a dependable reagent for their critical work in protein science and general biochemistry. The consistent Bicine biological buffer pH range offered by the product makes it a valuable asset for any research laboratory.