Tricine, identified by CAS 5704-04-1, stands out as a critical buffer component in diverse biological research fields. Developed by Ulrich Good, it is part of a series of buffering agents designed for biological applications, minimizing interference with cellular processes. Its primary function is to maintain a stable pH, a fundamental requirement for enzyme activity, protein stability, and overall cellular function. The chemical structure of Tricine, N-[Tris(hydroxymethyl)methyl]glycine, imparts unique buffering properties that are highly valued in advanced scientific endeavors.

One of the most significant tricine electrophoresis buffer applications is in specialized SDS-PAGE protocols. This buffer system is particularly adept at separating proteins and peptides in the low molecular weight range (1-100 kDa). Traditional buffers can struggle with resolving these smaller molecules effectively, leading to band smearing or poor resolution. Tricine-based systems overcome these limitations, providing sharper bands and clearer separation, which is crucial for downstream analyses such as Western blotting or mass spectrometry. The meticulous tricine buffer preparation ensures optimal performance in these demanding assays.

The utility of Tricine extends beyond electrophoresis. In biochemistry, its ability to stabilize pH in the slightly alkaline range (pH 7.4-8.8) makes it a valuable buffer for enzyme assays and other biochemical reactions where precise pH control is essential. Researchers often inquire about what Tricine is used for in biochemistry, and its role in maintaining optimal reaction conditions is a key answer. Furthermore, its application in sample preparation for mass spectrometry contributes to the cleaner ion profiles and more accurate data, making high purity Tricine for research indispensable.

For scientists looking to integrate Tricine into their research workflows, understanding its sourcing is important. When you consider to buy Tricine, opting for a reputable supplier like NINGBO INNO PHARMCHEM CO.,LTD. guarantees that you receive a product meeting stringent quality standards, such as Purity Degree 99% Tricine CAS No. 5704-04-1. This ensures reproducibility and reliability in your experimental outcomes. The science behind Tricine highlights its indispensable nature in advancing biological research, from fundamental protein studies to complex molecular analyses.