At NINGBO INNO PHARMCHEM CO.,LTD., we understand the vital importance of high-quality reagents in advancing scientific research. Nitrotetrazolium Blue Chloride, identified by its CAS number 298-83-9, stands out as a key compound widely utilized for its efficacy as a substrate in numerous enzyme activity assays. Its ability to participate in colorimetric reactions makes it an invaluable tool for researchers seeking to quantify or visualize the activity of specific enzymes.

One of the primary applications of Nitrotetrazolium Blue Chloride is its function as a substrate for dehydrogenases and other peroxidases. This characteristic is particularly leveraged in the determination of enzymes like glucose-6-phosphate dehydrogenase. This enzyme plays a critical role in cellular metabolism, and its accurate measurement is essential for understanding various physiological and pathological conditions. By providing a reliable supply of high-purity Nitrotetrazolium Blue Chloride, we empower laboratories to conduct these crucial diagnostic tests with confidence. When you buy this product from us, you are investing in precision and consistency for your laboratory's needs.

Furthermore, Nitrotetrazolium Blue Chloride serves as an excellent substrate for alkaline phosphatase. Upon enzymatic action, it catalyzes the formation of insoluble blue products, which can be readily detected and localized. This property makes it exceptionally useful in techniques such as immunohistochemistry (IHC) and immunocytochemistry (ICC). In these methods, the enzyme conjugated to an antibody is detected by its activity on a substrate like NTB-Cl, resulting in a localized blue precipitate that highlights the presence and distribution of the target antigen within cells and tissues. The price for such critical reagents reflects their importance in research outcomes.

The utility of Nitrotetrazolium Blue Chloride extends to western blotting (WB) assays as well. Here, it aids in the detection of specific proteins that have been coupled with enzymes capable of reacting with the substrate. The resulting color change provides a visual indicator of protein presence and relative abundance. For laboratories looking to purchase these essential biochemical reagents, partnering with a reputable supplier like NINGBO INNO PHARMCHEM CO.,LTD. ensures access to products that meet stringent quality standards. Our commitment as a manufacturer in China means we can offer competitive pricing for these vital research chemicals.

In summary, Nitrotetrazolium Blue Chloride is a versatile and indispensable reagent in modern biochemical and biological research. Whether it's for accurately determining enzyme activity, enhancing diagnostic imaging in IHC/ICC, or facilitating protein detection in western blots, its role is paramount. We encourage researchers and diagnostic labs to consider our offerings for their next purchase of this critical compound, confident in the quality and reliability we provide.