In the dynamic world of biochemistry and molecular biology, precise control over experimental conditions is paramount. Among the essential tools for researchers are buffers, which maintain stable pH levels crucial for enzyme activity, protein stability, and cellular processes. One such indispensable buffer is ADA, chemically known as N-(2-Acetamido)iminodiacetic Acid (CAS: 26239-55-4). NINGBO INNO PHARMCHEM CO.,LTD. is proud to highlight the significance of this compound and its role in advancing scientific research.

ADA buffer is a member of the renowned 'Good's Buffers,' a series of zwitterionic compounds developed to offer superior buffering capacity in specific pH ranges relevant to biological systems. The key advantage of ADA lies in its pKa value, which translates to an effective buffering range typically between pH 6.0 and 7.2. This specific range makes it ideal for experiments that require a neutral to slightly acidic pH, common in many cellular and enzymatic studies. Researchers often purchase ADA for its reliable performance in these conditions.

One of the notable features of N-(2-Acetamido)iminodiacetic Acid is its ability to prevent the oxidation and irreversible denaturation of proteins. This protective characteristic is particularly valuable during gel electrophoresis, a fundamental technique for separating proteins based on size and charge. By using ADA, scientists can ensure that their protein samples remain intact and their structures are preserved, leading to more accurate and interpretable results. For those looking to buy ADA, NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality ADA buffer that meets stringent research standards.

Beyond its protein-stabilizing properties, ADA also functions as an effective chelator for various metal ions. It has been shown to complex with ions such as Manganese (Mn(II)), Copper (Cu(II)), Nickel (Ni(II)), Zinc (Zn(II)), and Cobalt (Co(II)) at or below physiological pH. This metal-chelating capability can be beneficial in controlling the availability of metal cofactors in enzymatic reactions or preventing metal-catalyzed degradation. Understanding the use of ADA as a metal ion chelator in biological systems is crucial for experimental design.

While ADA is a powerful tool, researchers should be aware of its potential to interfere with certain biochemical assays. Specifically, studies indicate that ADA may affect the color development in bicinchoninic acid (BCA) assays, a common method for protein quantitation. Therefore, when planning experiments involving both ADA buffering and BCA assays, careful consideration and potentially alternative assay methods are recommended. This highlights the importance of understanding the comprehensive profile of any chemical supplier's product.

Furthermore, ADA finds application in preparing immobilized pH gradients (IPGs), which are critical for high-resolution protein separation techniques like isoelectric focusing. By immobilizing the buffering capacity onto a matrix, IPGs allow for the precise separation of proteins based on their isoelectric points. The availability of N-(2-Acetamido)iminodiacetic Acid from reliable manufacturers like NINGBO INNO PHARMCHEM CO.,LTD. supports the development of advanced separation technologies.

In summary, N-(2-Acetamido)iminodiacetic Acid is a versatile and valuable biochemical reagent. Its robust buffering capacity within the pH 6.0-7.2 range, protein-stabilizing properties, and metal-chelating abilities make it an essential component in many biological research protocols. For scientists seeking to purchase ADA or explore its applications, NINGBO INNO PHARMCHEM CO.,LTD. stands as a trusted supplier, committed to providing high-quality chemicals to support your scientific endeavors.