Phenyl Isothiocyanate (PITC), identified by its CAS number 103-72-0, is a chemical compound of significant importance in both academic research and industrial applications. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality PITC, recognizing its dual role as a crucial reagent in precise biochemical analysis and a versatile intermediate in organic synthesis.

Historically, PITC gained prominence for its role in the Edman degradation method, a technique fundamental to protein sequencing. In this process, PITC reacts with the N-terminal amino group of a peptide under alkaline conditions. Subsequent acid treatment cleaves the modified N-terminal amino acid, which can then be identified. This reliable reaction underpins the ability to perform accurate peptide sequencing with Phenyl Isothiocyanate, a process vital for understanding protein function and structure.

Beyond its application in sequencing, PITC is extensively used in conjunction with High-Performance Liquid Chromatography (HPLC) for the analysis of amino acids. PITC acts as a derivatizing agent, reacting with amino acids to form derivatives that are easily detected by HPLC systems. This capability is crucial for quantitative amino acid analysis by HPLC, supporting quality control in food science, pharmaceuticals, and biochemical research. NINGBO INNO PHARMCHEM CO.,LTD. supplies PITC that meets stringent purity requirements for these sensitive analytical applications.

The synthetic utility of PITC is equally noteworthy. Its reactive isothiocyanate group allows it to serve as a building block in various organic synthesis pathways. It can react with amines, alcohols, and other nucleophiles to form a diverse range of compounds, including heterocyclic molecules and modified peptides. This makes PITC a valuable reagent for NINGBO INNO PHARMCHEM CO.,LTD. in their efforts to synthesize novel pharmaceutical intermediates and specialty chemicals. Its function as a derivatizing reagent for amines highlights its broad reactivity profile.

In summary, Phenyl Isothiocyanate is a multifaceted chemical that supports critical scientific endeavors. Its established role in peptide sequencing and amino acid analysis, coupled with its utility in organic synthesis, underscores its value. NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to providing researchers and manufacturers with reliable access to high-purity PITC, facilitating advancements in chemistry and life sciences.