In the realm of scientific research, certain chemical compounds stand out for their multifaceted utility and critical role in advancing our understanding of biological and chemical processes. Among these is 2,3,5-Triphenyltetrazolium Chloride (TTC), identified by its CAS number 298-96-4. This compound, a vital tetrazolium salt, has garnered significant attention for its applications ranging from visualizing cellular activity to acting as a precise analytical reagent.

One of the most prominent uses of 2,3,5-Triphenyltetrazolium Chloride is within cell biology. Here, it functions as a vital stain, often referred to as a redox indicator. When TTC encounters metabolically active cells, particularly those with active dehydrogenases, it is reduced to a red insoluble formazan. This color change provides a clear visual cue to the viability and metabolic state of cells. Researchers frequently employ this 2,3,5-triphenyltetrazolium chloride for cell biology studies to assess cell health, screen for cytotoxic compounds, or map tissue viability. The ability to discern active metabolic regions within tissues or cell cultures makes it an indispensable tool.

Beyond its role in visual biological assays, 2,3,5-Triphenyltetrazolium Chloride also shines as an analytical and chromatographic reagent. Its distinct chemical properties allow it to react selectively with certain substances, making it valuable for qualitative and quantitative analysis. For instance, it serves as a sensitive reagent for reducing sugar detection. By observing the colorimetric reaction, scientists can identify and quantify reducing sugars in various samples, which is crucial in food science, clinical diagnostics, and metabolic studies. Furthermore, it aids in distinguishing between ethanol, ketones, and simple aldehydes, providing a means for differentiation in complex mixtures.

The compound's utility extends to the precise determination of enzyme activity, particularly for dehydrogenases. Many biochemical pathways rely on the action of these enzymes, and monitoring their activity is key to understanding cellular respiration and metabolic flux. The reaction of 2,3,5-Triphenyltetrazolium Chloride with NADH or NADPH, coenzymes produced during these enzymatic processes, results in the formation of the colored formazan. This makes it an excellent tool for measuring dehydrogenase activity, allowing researchers to gain insights into metabolic rates and potential enzyme deficiencies or enhancements. The ability to precisely measure dehydrogenase activity is paramount in fields like enzymology and diagnostics.

Moreover, the compound finds application in pesticide residue analysis. Its reactivity and ability to form colored products can be leveraged in specific analytical methods designed to detect trace amounts of certain agricultural chemicals. This contributes to ensuring food safety and environmental monitoring.

For laboratories looking to procure high-quality 2,3,5-Triphenyltetrazolium Chloride, NINGBO INNO PHARMCHEM CO.,LTD. offers a reliable source. Ensuring access to a dependable supplier for your 2,3,5-triphenyltetrazolium chloride purchase is vital for consistent research outcomes. The availability of this reagent facilitates advancements in various scientific disciplines, underlining its importance in modern research laboratories.