O-Phthalaldehyde (OPA), identified by its CAS number 643-79-8, is a molecule renowned for its dual utility in high-level disinfection and sophisticated biochemical analysis. While its disinfecting properties are widely recognized, its application as a sensitive fluorogenic reagent for assaying amines, particularly in the analysis of amino acids, peptides, and proteins, is equally significant. NINGBO INNO PHARMCHEM CO.,LTD. highlights these crucial biochemical roles.

The magic of OPA in biochemical assays lies in its rapid reaction with primary amines in the presence of thiols, typically producing highly fluorescent derivatives. This reaction allows for the sensitive detection and quantification of amino acids, which are the building blocks of proteins. The OPA reagent for amino acid analysis enables researchers to accurately measure protein content and study amino acid profiles in various biological samples, from cell cultures to clinical diagnostics. The specificity of this reaction is a key factor in its widespread adoption in chromatography and capillary electrophoresis techniques.

Understanding the CAS 643-79-8 chemical properties is essential to appreciating its analytical power. Unlike some other derivatization agents, OPA reactions are fast and typically carried out under mild conditions, often at room temperature. This 'ready-to-use' nature simplifies experimental procedures, a benefit that laboratories globally appreciate. The fluorescence intensity generated is directly proportional to the concentration of the amine, allowing for precise quantitative measurements.

The comparison between O-Phthalaldehyde vs glutaraldehyde advantages also extends to analytical applications. While glutaraldehyde has its uses, OPA’s high fluorescence quantum yield and specific reactivity make it superior for quantitative amino acid analysis. This makes OPA a more efficient and informative tool for researchers studying biological processes. The ability to easily buy high purity O-Phthalaldehyde online ensures that laboratories have access to this critical reagent for their cutting-edge research.

The role of OPA as a pharmaceutical intermediate also connects to its chemical versatility. Its structural features allow it to be incorporated into more complex molecules, which can have therapeutic applications. NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to supplying OPA that meets the stringent quality requirements for both analytical and synthetic purposes, supporting innovation across scientific disciplines.