Hypoxanthine, identified by its CAS number 68-94-0, is a molecule that bridges the gap between fundamental biochemistry and practical laboratory applications, most notably in the field of cell culture. Its role as a nutrient additive and its involvement in specific biochemical pathways make it an essential component in many cell culture media formulations. Understanding the precise aspects of hypoxanthine cell culture use is vital for researchers aiming to optimize cell growth, viability, and experimental outcomes.

As a purine derivative, hypoxanthine is a precursor for purine synthesis, a process that is fundamental for the creation of DNA and RNA. This makes it a critical nutrient for cells that may have limited ability to synthesize purines endogenously, such as certain immortalized cell lines or specialized cell types used in biotechnology. Its inclusion in culture media ensures that these cells have access to the necessary building blocks for proliferation and cellular function. Researchers often rely on detailed information about hypoxanthine biochemical research to tailor media compositions for specific cell lines or experimental conditions.

Beyond general nutritional support, hypoxanthine plays a more specialized role in certain experimental methodologies. For instance, it is used in conjunction with other compounds in selection media for hybridoma technologies, which are crucial for the production of monoclonal antibodies. In these scenarios, hypoxanthine, along with other reagents like thymidine and aminopterin (forming HAT medium), selectively supports the growth of hybridoma cells while inhibiting the growth of unfused myeloma or spleen cells. This targeted application highlights the versatility of hypoxanthine as a research tool.

Furthermore, hypoxanthine's involvement in metabolic pathways makes it relevant for studies investigating cellular responses to various stresses, including hypoxia. While not directly a cell culture application in the traditional sense, the understanding derived from hypoxanthine in hypoxia ischemia studies can inform the design of cell culture experiments aimed at mimicking these conditions in vitro. The availability of high-quality hypoxanthine from reliable sources, such as leading hypoxanthine suppliers china offers, ensures that researchers have consistent access to this critical compound for their experiments.

In conclusion, hypoxanthine (CAS 68-94-0) is a compound with multifaceted importance in cell biology and laboratory research. Its dual role as a crucial nutrient and a sophisticated research tool in specialized applications underscores its value in advancing scientific understanding and biotechnological applications. Ensuring access to pure and well-characterized hypoxanthine is key to the success of countless cellular and biochemical investigations.