In scientific laboratories focused on health, nutrition, and pharmaceutical development, the ability to accurately measure the antioxidant capacity of compounds is crucial. Among the array of analytical tools available, DPPH, scientifically known as 2,2-Diphenyl-1-picrylhydrazyl (CAS 1898-66-4), stands out as a cornerstone reagent. This stable free radical is instrumental in a variety of assays due to its predictable behavior and easily measurable color change.

The fundamental principle behind DPPH's utility lies in its function as a DPPH free radical scavenger. In solution, DPPH exhibits a deep violet hue, indicative of its unpaired electron. When exposed to compounds that can donate hydrogen atoms, such as antioxidants, DPPH is reduced to its non-radical form, DPPH-H. This reduction results in a loss of the violet color, transforming into a pale yellow. The degree of color decolorization is directly proportional to the antioxidant activity of the sample being tested. This makes the antioxidant assay DPPH one of the most common and reliable methods for screening and quantifying the antioxidant potential of diverse substances, from plant extracts to synthesized molecules.

The consistent performance of DPPH as a hydrogen radical scavenger compound makes it an invaluable tool for researchers seeking to understand oxidative stress and develop protective agents. Its application is not limited to antioxidant assays; DPPH also plays a role as a standard in Electron Paramagnetic Resonance (EPR) spectroscopy. Serving as an electron paramagnetic resonance (EPR) signal standard, it helps researchers calibrate their instruments, ensuring the accuracy and reproducibility of studies involving free radicals. This dual functionality highlights the broad applicability and scientific significance of the DPPH CAS 1898-66-4 uses.

Understanding DPPH's chemical behavior is key to mastering radical assays. Its stability as a free radical and its clear colorimetric response provide a straightforward yet powerful method for scientific inquiry. Whether you are evaluating the efficacy of a new dietary supplement, developing a more stable cosmetic formulation, or conducting fundamental research into the mechanisms of oxidative damage, DPPH is an essential reagent to consider.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing the scientific community with high-purity DPPH, ensuring that researchers have access to reliable tools for their critical work. Our goal is to support your efforts in uncovering the power of antioxidants and advancing scientific knowledge.