In the pharmaceutical industry, the quality of raw materials and intermediates directly impacts the safety and efficacy of the final drug product. N-Cbz-D-Alanine (CAS 26607-51-2), a vital chiral building block, demands stringent quality control. At NINGBO INNO PHARMCHEM CO.,LTD., we employ a suite of advanced analytical techniques to ensure the purity, identity, and stereochemical integrity of our N-Cbz-D-Alanine batches.

A cornerstone of our quality assurance is N-Cbz-D-Alanine NMR characterization. Proton and Carbon-13 NMR spectroscopy provide detailed structural information, confirming the presence of the Cbz group and the alanine backbone. Characteristic peaks for the phenyl protons, benzylic methylene, alpha-proton, and methyl group are meticulously analyzed. Discrepancies are investigated using 2D NMR techniques for unambiguous assignments, ensuring the correct molecular structure.

Crucially, the D-chirality of N-Cbz-D-Alanine must be verified. For this, N-Cbz-D-Alanine chiral HPLC analysis is indispensable. By utilizing specialized chiral stationary phases, we can separate and quantify enantiomers, determining the enantiomeric excess (ee%). This measurement is critical for preventing the contamination of the desired D-isomer with its L-enantiomer, a common issue in D-alanine protection Cbz group synthesis that can affect biological activity.

Furthermore, High-Resolution Mass Spectrometry (HRMS) is employed to confirm the exact molecular weight of N-Cbz-D-Alanine, validating its elemental composition and identifying any trace impurities. Infrared (IR) spectroscopy complements these methods by confirming the presence of key functional groups, such as the carbamate and carboxylic acid carbonyls. Melting point determination also serves as a quick indicator of purity, with a narrow and sharp melting range signifying a highly pure compound.

Understanding the N-Cbz-D-Alanine synthesis mechanisms and implementing these rigorous analytical checks are integral to our commitment at NINGBO INNO PHARMCHEM CO.,LTD. We are dedicated to providing high-purity N-Cbz-D-Alanine that meets the demanding requirements of the pharmaceutical and chemical research sectors, ensuring reliability and consistency from synthesis to application.