The Science Behind Avidin: A Deep Dive into Biotin Interaction
Avidin (CAS 1405-69-2) is a tetrameric protein celebrated for its extraordinary affinity and specificity for biotin (Vitamin B7). This unique molecular partnership is the bedrock of its widespread use in biotechnology, diagnostics, and research. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality Avidin, understanding that its efficacy hinges on the integrity of this fundamental biological interaction.
The science underpinning the Avidin biotin binding applications is truly remarkable. Each Avidin molecule possesses four identical subunits, and each subunit can bind one molecule of biotin with an extremely high affinity (Kd approximately 10^-15 M). This tight and specific interaction is characterized by its near-irreversibility under physiological conditions, meaning the Avidin-biotin complex is exceptionally stable. This stability is crucial for applications requiring robust and long-lasting detection or capture systems, making it a preferred choice when you buy specialized reagents.
This high affinity and specificity make Avidin an ideal component for Avidin in diagnostic reagents. In many diagnostic kits, biotinylated antibodies or antigens are used. When these are detected using an Avidin conjugate (e.g., Avidin conjugated to an enzyme like horseradish peroxidase), the strong Avidin-biotin interaction ensures that the label is tightly bound to the target molecule, leading to highly sensitive and specific detection of analytes. The price reflects the complex manufacturing and purification processes required to achieve this high performance.
As a key pharmaceutical intermediate, Avidin's applications extend to various research methodologies. In affinity chromatography, biotinylated molecules are immobilized onto Avidin-coated beads or columns for highly efficient purification. This technique is essential for isolating pure proteins for study or therapeutic development. The consistent availability and quality of Avidin are therefore critical for researchers in these demanding fields. The purchase of Avidin from reliable suppliers ensures predictable experimental outcomes.
NINGBO INNO PHARMCHEM CO.,LTD. is committed to supplying Avidin that adheres to rigorous quality standards. We ensure that our product maintains the inherent strength and specificity of the Avidin-biotin interaction, providing researchers and manufacturers with a dependable pharmaceutical intermediate. Our dedication to quality supports your efforts to buy reliable reagents for your scientific endeavors.
In summary, the exceptional science behind Avidin's biotin interaction, characterized by its high affinity, specificity, and stability, makes Avidin CAS 1405-69-2 an indispensable tool across a multitude of biological applications.
Perspectives & Insights
Agile Reader One
“This tight and specific interaction is characterized by its near-irreversibility under physiological conditions, meaning the Avidin-biotin complex is exceptionally stable.”
Logic Vision Labs
“This stability is crucial for applications requiring robust and long-lasting detection or capture systems, making it a preferred choice when you buy specialized reagents.”
Molecule Origin 88
“This high affinity and specificity make Avidin an ideal component for Avidin in diagnostic reagents.”