The efficiency and accuracy of protein analysis techniques, such as Western blotting, are heavily reliant on the quality of reagents used, particularly the transfer buffers. Understanding the properties of these buffers is crucial for researchers aiming for optimal results. This article explores the scientific basis behind protein transfer and highlights the significant advantages offered by CAPSO Sodium Salt (CAS 102601-34-3), a leading zwitterionic buffer for high pH applications. NINGBO INNO PHARMCHEM CO.,LTD., a trusted chemical manufacturer in China, is proud to supply this essential research compound.

Protein transfer, also known as electroblotting, is a fundamental step in Western blotting, where proteins separated by gel electrophoresis are transferred to a solid membrane support for subsequent detection. The effectiveness of this transfer process is influenced by several factors, including the buffer system's pH. Conventional buffers like Tris-Glycine, while effective for many proteins, often struggle with the transfer of highly basic proteins (those with a pI above 8.5) due to charge repulsion or suboptimal conditions. This is where buffers like CAPSO Sodium Salt come into play.

CAPSO Sodium Salt provides a stable buffering environment in the alkaline pH range of 8.9-10.3. This elevated pH is crucial for facilitating the efficient transfer of positively charged (basic) proteins towards the cathode. By maintaining a consistent high pH, CAPSO Sodium Salt helps to overcome the limitations of lower pH buffers, ensuring that a broader range of proteins can be successfully blotted. For researchers involved in protein identification, this capability is invaluable, as it allows for a more complete analysis of protein expression profiles.

The zwitterionic nature of CAPSO Sodium Salt contributes to its minimal interaction with biological molecules, including proteins and enzymes. This characteristic is vital for ensuring that the buffer itself does not interfere with the integrity or function of the proteins being analyzed. Its high water solubility and stability at room temperature also simplify its use in laboratory settings. As a premier biological buffer for western blotting, CAPSO Sodium Salt has proven its worth in numerous studies.

NINGBO INNO PHARMCHEM CO.,LTD., as a dedicated high purity buffer supplier China, ensures that the CAPSO Sodium Salt we provide adheres to the highest quality standards. Our commitment to excellence means that researchers can rely on our products for consistent performance in critical applications. Whether you are working on basic biochemical research or advanced biopharmaceutical development, the right buffer can make a significant difference. By utilizing CAPSO Sodium Salt, scientists can achieve more efficient protein transfer and more accurate analytical results, ultimately accelerating scientific discovery.