Histochemistry and immunohistochemistry are indispensable fields in biological research and diagnostics, allowing scientists to visualize and localize specific molecules within cells and tissues. Central to many of these techniques is the use of enzyme substrates that produce a visible signal. NINGBO INNO PHARMCHEM CO.,LTD. is proud to offer Disodium 5-bromo-4-chloro-1H-indol-3-yl Phosphate (CAS: 102185-33-1), a high-quality reagent that significantly enhances cellular and tissue analysis.

Disodium 5-bromo-4-chloro-1H-indol-3-yl Phosphate, often abbreviated as BCIP, is a derivative of indole that serves as an excellent chromogenic substrate for the enzyme alkaline phosphatase (AP). In histochemical staining, AP is frequently conjugated to antibodies or probes that specifically bind to cellular components or antigens of interest. When BCIP is introduced to the sample, the AP enzyme catalyzes its hydrolysis, yielding an insoluble, colored product. This localized precipitation marks the precise location of the target molecule, providing invaluable spatial information about biological processes.

The utility of BCIP in immunohistochemistry (IHC) is particularly noteworthy. IHC allows for the identification and localization of specific proteins within tissue sections. By using an antibody against a target protein, which is then detected by an AP-conjugated secondary antibody and visualized with BCIP, researchers can map protein expression patterns in healthy and diseased tissues. This is critical for understanding cellular function, identifying biomarkers, and diagnosing various conditions. The sharp resolution offered by BCIP-based staining contributes to accurate interpretation of tissue morphology and cellular events.

Furthermore, BCIP finds application in cytochemistry and in situ hybridization (ISH). In ISH, it is used to detect specific nucleic acid sequences within cells, aiding in the study of gene expression and localization. The ability to perform such precise analyses relies heavily on the availability of reliable and sensitive chromogenic substrates. For scientists seeking to buy BCIP, NINGBO INNO PHARMCHEM CO.,LTD. ensures a product that meets stringent quality requirements for these demanding histochemical applications.

The efficacy of BCIP as a substrate is often amplified when used in conjunction with a secondary chromogen like Nitro Blue Tetrazolium (NBT). This BCIP/NBT combination produces a deeper, more intensely colored precipitate, further improving the visibility of target molecules, especially in challenging samples. The combination ensures enhanced sensitivity and clarity, making it a preferred choice for many histochemical protocols.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to supporting the scientific community by providing essential biochemical intermediates. Our Disodium 5-bromo-4-chloro-1H-indol-3-yl Phosphate is a testament to this commitment, offering the purity and performance necessary for advanced histochemical and immunohistochemical analyses. By choosing our products, researchers gain access to tools that can unlock deeper insights into cellular and molecular biology.