In the critical field of agriculture and seed science, determining seed viability is a fundamental step. The TTC test for seed viability has emerged as a highly effective and widely adopted method for this purpose. At its core is 2,3,5-Triphenyltetrazolium Chloride (TTC), identified by CAS 298-96-4, a chemical that provides a clear visual indication of germination potential. NINGBO INNO PHARMCHEM CO.,LTD. is a key supplier of this vital reagent, supporting agricultural research and industry.

The principle behind the TTC seed viability test is rooted in the metabolic activity of dormant embryos within seeds. Viable seeds possess active respiratory systems and associated enzymes, such as dehydrogenases. These enzymes reduce TTC to a red-colored formazan compound. Seeds with healthy embryos will stain a deep red, indicating viability, while dead or dormant seeds with inactive embryos will remain unstained or show minimal coloration.

This method offers significant advantages over traditional germination tests, which can be time-consuming. The TTC assay provides rapid results, often within hours, allowing for quicker decision-making in seed quality control, breeding programs, and seed storage management. The clarity of the red staining also makes interpretation straightforward.

Beyond its prominent role in seed testing, TTC continues to be a valuable redox indicator in biochemical experiments. Its use in assessing cellular respiration and enzyme activity in various biological samples remains a cornerstone application. Whether studying plant physiology or animal cell metabolism, TTC provides a reliable way to gauge biological activity.

As an analytical reagent for reducing sugars and for detecting dehydrogenase activity, TTC demonstrates its broad utility in chemical and biological laboratories. Its consistent performance makes it a trusted reagent for quantitative and qualitative analyses across different scientific disciplines.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing researchers and industry professionals with high-quality 2,3,5-Triphenyltetrazolium Chloride. We understand the importance of a reliable and pure reagent for accurate seed viability testing and other critical applications. Partner with NINGBO INNO PHARMCHEM CO.,LTD. for your TTC supply and ensure the success of your vital research and quality control processes.