Disodium 5-bromo-4-chloro-1H-indol-3-yl Phosphate, scientifically identified by CAS number 102185-33-1, is a critical reagent in modern life science research. At NINGBO INNO PHARMCHEM CO.,LTD., we focus on delivering high-purity biochemicals, and BCIP is a prime example of a compound with extensive and impactful applications. Understanding its properties and how to utilize it effectively is key for researchers working in molecular biology, diagnostics, and biochemistry.

Chemically, BCIP is an organic compound derived from the indole nucleus. Its structure, featuring bromine and chlorine atoms on the indole ring and a phosphate group attached at the 3-position, is essential for its function as a substrate for alkaline phosphatase (AP). When AP enzymes act on BCIP, they release the phosphate group, leading to the formation of 5-bromo-4-chloro-3-hydroxyindole. This intermediate molecule then undergoes oxidation and dimerization, typically in the presence of an oxidizing agent like Nitro Blue Tetrazolium (NBT), to form an intensely colored, insoluble precipitate. The most common colors observed are blue or purple, depending on the specific reaction conditions and co-substrates used.

The primary application of BCIP is as a chromogenic substrate for alkaline phosphatase. This enzyme is frequently used as a label in various immunoassay techniques due to its stability and catalytic efficiency. In applications such as Western blotting, Northern blotting, Southern blotting, immunohistochemistry (IHC), and in situ hybridization (ISH), BCIP provides a visual output for the presence and location of the enzyme-labeled molecule. The sensitivity and specificity of the BCIP-based detection system make it invaluable for researchers needing to identify target proteins or nucleic acids with high accuracy.

The solubility of BCIP in water is a practical advantage, allowing for easy preparation of stock solutions. However, it is sensitive to moisture and light, necessitating proper storage conditions—typically at -20°C in a dry, well-sealed container—to maintain its stability and efficacy. Researchers often seek to buy BCIP from reliable suppliers to ensure product integrity for critical experiments.

At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to providing the scientific community with essential biochemical intermediates of the highest quality. Our Disodium 5-bromo-4-chloro-1H-indol-3-yl Phosphate is rigorously tested to ensure purity and consistent performance, empowering researchers to achieve reliable and reproducible results. Whether you are optimizing existing protocols or developing new detection strategies, incorporating high-quality BCIP is a key step towards successful outcomes. We invite you to explore our product offerings and discover how our chemical expertise can support your research needs.

The continued reliance on chromogenic detection methods in life sciences underscores the importance of compounds like BCIP. Its versatile utility and the clear signals it generates make it a fundamental tool for advancing scientific understanding across numerous disciplines.