NINGBO INNO PHARMCHEM CO.,LTD. is a premier supplier of specialty chemicals, with a particular focus on reagents for peptide synthesis. Today, we shed light on Fmoc-His(Fmoc)-OH, a fundamental Fmoc-protected amino acid that is indispensable for researchers engaged in Solid-Phase Peptide Synthesis (SPPS). Understanding its function and benefits is key to optimizing peptide production.

Peptide synthesis, the process of creating peptide molecules, is a cornerstone of modern biotechnology and pharmaceutical research. SPPS, a technique that allows for the stepwise assembly of amino acids on a solid support, has been significantly advanced by the development of orthogonal protecting group strategies. Among these, the Fmoc (9-fluorenylmethoxycarbonyl) group is widely favored for its base-labile nature, which allows for selective removal without harsh acidic conditions. For amino acids like histidine, which possess reactive side chains, effective protection is crucial to prevent unwanted side reactions and maintain the integrity of the growing peptide chain.

Fmoc-His(Fmoc)-OH is a derivative of histidine where both the alpha-amino group and the imidazole side chain are protected by the Fmoc group. This dual protection is vital for histidine, as its imidazole ring can lead to epimerization or participate in various undesirable reactions during peptide coupling steps. By utilizing Fmoc-His(Fmoc)-OH, chemists can ensure that the histidine residue is incorporated accurately and efficiently into the peptide sequence. This makes it a sought-after component among Fmoc-amino acid derivatives for SPPS, contributing to more reliable synthesis outcomes.

The advantages of using Fmoc-His(Fmoc)-OH are manifold. It contributes to higher purity of the final peptide product and improves the overall yield of the synthesis. This is particularly important for researchers working on therapeutic peptides or complex protein fragments where even minor impurities can affect biological activity. The ease of handling and compatibility with standard Fmoc deprotection protocols (typically using piperidine) further underscore its utility. Access to reliable custom peptide synthesis reagents and building blocks like Fmoc-His(Fmoc)-OH is a significant enabler for scientific discovery.

NINGBO INNO PHARMCHEM CO.,LTD. is committed to providing the scientific community with superior quality reagents. Our Fmoc-His(Fmoc)-OH is produced to high standards, ensuring consistent performance in your peptide synthesis endeavors. By partnering with us, researchers gain access to essential building blocks that facilitate groundbreaking work in biochemistry, medicine, and materials science. Trust NINGBO INNO PHARMCHEM CO.,LTD. for your peptide synthesis reagent needs.