Understanding Glycosidase Activity with 4-Nitrophenyl Alpha-D-Glucopyranoside
NINGBO INNO PHARMCHEM CO.,LTD. is dedicated to supplying researchers with the tools they need to unravel complex biological processes. One such essential tool is 4-Nitrophenyl Alpha-D-Glucopyranoside (CAS 3767-28-0), a highly regarded chromogenic substrate for alpha-D-glucosidase. This compound is pivotal for accurate enzyme activity measurement, especially when studying enzymes like yeast alpha-D-glucosidase.
The mechanism by which 4-Nitrophenyl Alpha-D-Glucopyranoside functions is straightforward yet powerful. In the presence of a suitable alpha-D-glucosidase, the glycosidic bond linking the nitrophenyl group to the glucose moiety is cleaved. This reaction liberates p-nitrophenol, which, under alkaline conditions, forms the p-nitrophenolate ion. This ion absorbs light maximally at around 400 nm, resulting in a visible yellow coloration. The intensity of this yellow color is directly proportional to the enzyme's activity, enabling quantitative analysis. The availability of this 4-nitrophenyl alpha-d-glucopyranoside enzyme substrate from NINGBO INNO PHARMCHEM CO.,LTD. ensures that researchers have a reliable method at their disposal.
Beyond its enzymatic applications, 4-Nitrophenyl Alpha-D-Glucopyranoside also serves as a valuable pharmaceutical intermediate. NINGBO INNO PHARMCHEM CO.,LTD. recognizes the importance of this compound in drug synthesis and provides it to the pharmaceutical industry with the assurance of high purity and consistent quality. For those looking to purchase chemical compounds for manufacturing, sourcing CAS 3767-28-0 from a reputable supplier is critical.
For scientists and manufacturers alike, understanding the detailed specifications and applications of chemicals like 4-Nitrophenyl Alpha-D-Glucopyranoside is key. NINGBO INNO PHARMCHEM CO.,LTD. provides comprehensive technical documentation and support, empowering their clients to achieve their research and production goals effectively.
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