Iodoacetamide (CAS 144-48-9) is a chemical compound widely recognized for its utility in biochemical research, particularly as an alkylating agent. Its unique properties make it invaluable for modifying proteins, specifically targeting cysteine residues. For researchers, understanding its characteristics, proper handling, and diverse applications is key to leveraging its full potential in experimental settings. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality Iodoacetamide for your research needs.

Iodoacetamide typically appears as a white crystalline solid. Its molecular formula is C2H4INO, and it has a molecular weight of approximately 185.0 g/mol. A key characteristic of Iodoacetamide is its reactivity towards nucleophiles, especially the thiol (-SH) groups of cysteine residues found in proteins. This reaction leads to the formation of a covalent thioether bond, effectively alkylating the cysteine. This specific reactivity is foundational for its use in various biochemical applications, including preventing disulfide bond formation and aiding in peptide mapping.

Proper handling and storage of Iodoacetamide are crucial due to its reactivity and potential sensitivity. It is often recommended to store Iodoacetamide in a cool place, protected from light, and in a tightly sealed container to maintain its stability and efficacy. When preparing solutions, it is advisable to do so immediately before use, as Iodoacetamide can be unstable in aqueous solutions over prolonged periods. Researchers should always consult the safety data sheet (SDS) for detailed information on handling precautions, personal protective equipment, and disposal methods.

The applications of Iodoacetamide in biochemical research are broad. It is extensively used in proteomics to modify cysteine residues, thereby preventing disulfide bond formation and ensuring the integrity of protein samples for analysis by techniques such as mass spectrometry or chromatography. This targeted modification is essential for accurate protein identification and characterization. Beyond proteomics, Iodoacetamide is employed in studies of protein folding, enzyme kinetics, and in chemical biology for probing protein structures and functions. Its role in preventing unintended cross-linking makes it a reliable choice for researchers focused on precise protein modification.

For scientists looking to purchase Iodoacetamide, sourcing from a reputable supplier like NINGBO INNO PHARMCHEM CO.,LTD. ensures that you receive a product of high purity and consistent quality. This is vital for reproducible experimental results. Whether you are engaged in complex proteomics workflows or fundamental biochemical investigations, Iodoacetamide remains an indispensable tool for manipulating and analyzing proteins with precision. Understanding its properties and adhering to best practices for its use will undoubtedly enhance the success of your research endeavors.