Electrophoresis is a foundational technique in molecular biology and biochemistry, enabling the separation of nucleic acids and proteins based on their size, charge, and conformation. The performance of any electrophoresis experiment hinges significantly on the quality and stability of the running buffer. MOPS sodium salt, a zwitterionic buffering agent, is a critical component in many electrophoresis buffer formulations, providing consistent and reliable results. NINGBO INNO PHARMCHEM CO.,LTD. offers high-grade MOPS sodium salt essential for these demanding applications.

The chemical properties of MOPS sodium salt (CAS 71119-22-7) make it an ideal buffer for electrophoresis. Its pKa of approximately 7.20 at 25°C allows for stable pH maintenance within the range commonly used for separating biomolecules. This stability is crucial because variations in pH can affect the net charge of DNA, RNA, or proteins, altering their migration patterns and thus compromising the accuracy of the separation. By using MOPS sodium salt electrophoresis buffer, researchers can ensure that their molecules move through the gel matrix predictably and efficiently.

A typical MOPS-based electrophoresis buffer might include MOPS sodium salt, along with other salts like sodium acetate and chelating agents like EDTA. For instance, a common buffer system for RNA electrophoresis uses MOPS, sodium acetate, and EDTA. The MOPS component buffers the system to a neutral pH, preventing RNA degradation that can occur in more alkaline conditions. The precise formulation ensures that the ionic strength and pH remain constant throughout the run, which is vital for obtaining sharp, well-defined bands.

Researchers often require specific buffer systems for different types of electrophoresis. Whether performing agarose gel electrophoresis for DNA or polyacrylamide gel electrophoresis (PAGE) for proteins, the ability to buy MOPS sodium salt and integrate it into custom buffer recipes provides flexibility. The high purity of MOPS sodium salt offered by NINGBO INNO PHARMCHEM CO.,LTD. guarantees that no interfering substances are introduced, which could otherwise lead to ghost bands or reduced sample integrity.

The stability provided by MOPS sodium salt is a key advantage over other buffering systems that might be more sensitive to changes in temperature or ionic concentration. This robustness ensures that experiments can be conducted with greater confidence, especially in large-scale or complex purification protocols where sample throughput is high. The ease of preparing and handling MOPS sodium salt solutions further contributes to its popularity among laboratory professionals.

In conclusion, MOPS sodium salt is a fundamental reagent for achieving excellence in electrophoresis. Its reliable pH buffering capabilities and broad compatibility with various electrophoresis techniques make it an essential component for any molecular biology laboratory. By sourcing high-quality MOPS sodium salt, researchers can optimize their running buffers, leading to more accurate, reproducible, and high-quality separation results. NINGBO INNO PHARMCHEM CO.,LTD. is proud to support these endeavors by providing top-tier chemical reagents.