MES as a Biological Buffer: Essential for Scientific Research
Scientific research hinges on precise control over experimental conditions, and maintaining a stable pH is fundamental to achieving reliable results. NINGBO INNO PHARMCHEM CO.,LTD. provides high-quality 4-Morpholineethanesulfonic Acid (MES), a compound widely recognized for its excellence as a biological buffer. With its unique properties, MES plays a critical role in various laboratory disciplines, from biochemistry to molecular biology.
MES, with its CAS number 4432-31-9, falls into the category of Good's buffers, a group of zwitterionic buffering agents developed for biological research. Its pKa value of approximately 6.15 at 25°C makes it particularly effective for maintaining pH in the range of 5.5 to 7.7, a crucial physiological pH range for many biological systems. This stability is vital for experiments involving enzymes, proteins, and nucleic acids, where even minor pH fluctuations can significantly impact their structure and function. The consistent availability of MES as a biological buffer is supported by dedicated 4-morpholineethanesulfonic acid supplier networks.
In biochemistry and molecular biology experiments, MES is frequently used in reagent solutions and for pH adjustment in media. For instance, it's employed in plant tissue culture to provide a stable environment for cell growth and proliferation. Its inertness towards various chemicals and enzymes ensures it doesn't interfere with the biological processes being studied. This makes it a preferred choice over other buffers that might exhibit unwanted reactivity.
Electrophoresis is another area where MES demonstrates significant value. In nucleic acid electrophoresis (DNA and RNA separation), MES can serve as a less toxic alternative to traditional buffers like TBE or TAE. It effectively separates nucleic acid fragments of varying sizes while minimizing damage to the genetic material. Similarly, in protein electrophoresis, such as SDS-PAGE and isoelectric focusing, MES helps maintain stable pH gradients, allowing for precise separation of proteins based on their molecular weight or isoelectric point. The purity of 4-morpholineethanesulfonic acid directly impacts the clarity of these separations.
Furthermore, MES finds application in chromatographic separations. As an anionic buffer, it is compatible with cation exchange chromatography and hydroxyapatite chromatography, assisting in the controlled elution and purification of target molecules. By working in tandem with stationary phases, MES helps optimize the separation process, leading to higher purity and yield of desired compounds.
The reliability and performance of MES as a biological buffer make it an indispensable tool for researchers worldwide. Companies like NINGBO INNO PHARMCHEM CO.,LTD. play a crucial role in ensuring that scientists have access to this high-quality chemical, supporting advancements in life sciences. Whether for routine lab work or cutting-edge research, the choice of MES underscores a commitment to experimental accuracy and reproducibility.
Perspectives & Insights
Alpha Spark Labs
“MES, with its CAS number 4432-31-9, falls into the category of Good's buffers, a group of zwitterionic buffering agents developed for biological research.”
Future Pioneer 88
“This stability is vital for experiments involving enzymes, proteins, and nucleic acids, where even minor pH fluctuations can significantly impact their structure and function.”
Core Explorer Pro
“The consistent availability of MES as a biological buffer is supported by dedicated 4-morpholineethanesulfonic acid supplier networks.”