Bis-Tris, a widely recognized zwitterionic buffer, offers more than just pH stabilization; it possesses intriguing chemical properties, including the ability to bind with metal ions. This characteristic, combined with its robust buffering capacity, makes it a compound of significant interest in various biochemical and molecular biology applications. Understanding these interactions is key to leveraging Bis-Tris effectively in research and development.

The core function of Bis-Tris as a buffer lies in its zwitterionic nature and its pKa of approximately 6.46. This allows it to maintain a stable pH between 5.8 and 7.2, a critical range for many biological systems. However, its interaction with metal ions adds another layer of utility. Research indicates that Bis-Tris exhibits a strong affinity for certain divalent metal cations, particularly copper (Cu²⁺) and lead (Pb²⁺). It also shows weaker binding to ions like magnesium (Mg²⁺), calcium (Ca²⁺), manganese (Mn²⁺), cobalt (Co²⁺), nickel (Ni²⁺), and zinc (Zn²⁺). This property can be advantageous in applications where controlling the concentration of free metal ions is important, such as in certain enzymatic assays or purification processes where metal ion contamination can be problematic. Exploring Bis-Tris metal ion binding can reveal novel experimental designs.

The buffering capacity itself is vital for numerous biological applications. For instance, in protein purification, the consistent pH provided by Bis-Tris helps maintain protein conformation and solubility, ensuring higher recovery rates. Its role in electrophoresis, acting as a catholyte, is another testament to its buffering prowess, contributing to the accurate separation of biomolecules. The Bis-Tris buffer pH range is well-suited for many of these sensitive procedures.

The chemical behavior of Bis-Tris extends to its role in preserving biological materials. Its ability to protect hemoglobin during freeze-drying highlights its protective qualities in challenging storage conditions. When considering the broader Bis-Tris uses in biochemistry, its multifaceted nature becomes apparent. From enzymatic studies to cell culture media, its buffering and chelating properties offer distinct advantages.

For researchers aiming to harness these properties, sourcing high-quality Bis-Tris is essential. NINGBO INNO PHARMCHEM CO.,LTD. provides Bis-Tris with guaranteed purity, supporting rigorous scientific investigation. Whether you are delving into the intricacies of Bis-Tris molecular biology applications or need a reliable buffer for routine lab work, understanding its chemical interactions, particularly its metal ion binding, can optimize your experimental outcomes. If you are looking to buy Bis-Tris, consider its specific properties for your application.