Bis-Tris: A Versatile Zwitterionic Buffer for Biochemistry and Bioprocessing
Discover the utility of Bis-Tris, an essential zwitterionic buffer for diverse life science applications.
Get a Quote & SampleProduct Core Value
Bis-Tris
Bis-Tris is a highly effective zwitterionic buffering agent renowned for its utility in molecular biology and biochemistry. It maintains a stable pH range crucial for various biological processes.
- Explore the effective buffering range of Bis-Tris, typically between pH 5.8 and 7.2, making it ideal for sensitive biological reactions.
- Learn about Bis-Tris uses in biochemistry, particularly in protein purification and biochemical assays where pH stability is paramount.
- Understand how Bis-Tris protects hemoglobin in freeze-drying applications, preserving its integrity and function.
- Discover the role of Bis-Tris in electrophoresis as a catholyte, contributing to efficient separation techniques.
Key Advantages
Broad pH Range Stability
The consistent pH buffering provided by Bis-Tris is essential for reproducible results in delicate assays and purification processes.
Bioprocessing Compatibility
With high purity options and GMP excipient grade availability, Bis-Tris is suitable for advanced biopharmaceutical manufacturing and cell culture applications.
Metal Ion Complexation
Bis-Tris exhibits notable binding affinities for specific metal ions, such as Cu and Pb, which can be advantageous in certain biochemical contexts.
Key Applications
Protein Purification
Utilize Bis-Tris in your protein purification workflows to maintain optimal pH conditions, ensuring protein stability and activity.
Electrophoresis
As a catholyte, Bis-Tris supports efficient separation in gel electrophoresis, contributing to clear banding and analysis.
Cell Culture
The stable pH environment created by Bis-Tris makes it a valuable component in cell culture media for research and bioproduction.
Biochemical Assays
Ensure the reliability of your biochemical assays by employing Bis-Tris for precise pH control throughout the experiment.