Bis-Tris, chemically known as 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, is a zwitterionic buffering agent that plays a significant role in various scientific disciplines, particularly in biochemistry and molecular biology. Its unique chemical structure grants it advantageous buffering capabilities within a specific pH range, making it indispensable for a multitude of experimental procedures and industrial applications.

The defining characteristic of Bis-Tris is its pKa value, typically around 6.46 at 25°C. This allows it to effectively buffer solutions in the pH range of 5.8 to 7.2. This pH range is physiologically relevant and critical for many biological processes, including enzymatic reactions, protein folding, and nucleic acid stability. Understanding the Bis-Tris buffer pH range is fundamental for its successful application. It is also known for its ability to complex with certain metal ions, notably copper (Cu) and lead (Pb), which can be a beneficial property in specific experimental setups.

The applications of Bis-Tris are extensive and varied. In biochemistry, it is widely used in protein purification, where maintaining a stable pH is essential for protein solubility and structural integrity. It also serves as a catholyte in electrophoresis, aiding in the separation of biomolecules. Another significant application is its use in protecting hemoglobin during freeze-drying processes, preserving its functionality. For researchers interested in the Bis-Tris uses in biochemistry, these applications highlight its versatility.

In the biopharmaceutical industry, Bis-Tris is recognized for its utility as an excipient and a processing aid. Its availability in high-purity, GMP-compliant grades makes it suitable for cell culture media, diagnostic reagents, and other critical bioprocessing steps. The consistency and reliability of Bis-Tris excipient grade products are crucial for ensuring the quality and safety of pharmaceutical products. Proper Bis-Tris storage and handling, typically at room temperature in a dry state, is recommended to maintain its optimal performance.

For scientists and manufacturers looking to source this essential buffer, quality and reliability are key considerations. NINGBO INNO PHARMCHEM CO.,LTD. is a reputable supplier offering Bis-Tris that meets stringent purity standards, suitable for demanding laboratory and industrial requirements. Exploring the various Bis-Tris molecular biology applications and understanding its benefits can guide researchers in selecting the right buffer for their specific needs. When you need to buy Bis-Tris, choosing a trusted supplier ensures you receive a product that consistently delivers the expected performance.