Peroxidase, a versatile enzyme with CAS 9003-99-0, is a cornerstone in the field of immunoassays, enabling sensitive and specific detection of biomolecules. Its catalytic activity is harnessed in techniques such as ELISA and Western blotting, making it an invaluable tool for researchers and diagnosticians. NINGBO INNO PHARMCHEM CO.,LTD. provides high-purity Peroxidase, supporting the critical applications of peroxidase in ELISA and other key immunoassays.

The fundamental principle behind using Peroxidase in immunoassays relies on its ability to catalyze the oxidation of chromogenic, fluorogenic, or chemiluminescent substrates, generating a measurable signal. In a typical ELISA, an antibody conjugated to Peroxidase is used. When this antibody binds to its target antigen, and the appropriate substrate is added, the Peroxidase enzyme triggers a reaction that produces a colored product. The intensity of this color is directly proportional to the amount of antigen present, allowing for quantitative analysis.

Similarly, in Western blotting, Peroxidase-conjugated secondary antibodies are used to detect specific proteins that have been transferred to a membrane. After incubation with a substrate, the Peroxidase produces a visible band at the location of the target protein, indicating its presence and size. This application is a prime example of peroxidase enzyme labeling in action.

The efficiency of peroxidase antibody conjugation is crucial for assay sensitivity. Researchers often utilize specialized kits to ensure stable and active conjugates. Beyond immunoassay applications, understanding what is peroxidase used for reveals its utility in areas like environmental remediation and even as a potential food additive, demonstrating its broad enzymatic capabilities.

NINGBO INNO PHARMCHEM CO.,LTD. supplies Peroxidase that meets the high standards required for these sensitive applications. The enzyme's stability and high turnover rate contribute to stronger signals and lower detection limits, which are critical for accurate results in biological research and clinical diagnostics. The development of innovative peroxidase labeling kits further simplifies experimental workflows.

While its role in laboratory techniques is paramount, it's worth noting that Peroxidase's broader applications, such as in peroxidase wastewater treatment, highlight its significant enzymatic versatility. However, for the scientific community focused on molecular detection, the precision offered by peroxidase in ELISA and similar assays remains its most impactful contribution. The ongoing exploration of peroxidase for biomolecules conjugation continues to push the boundaries of diagnostic sensitivity.

At NINGBO INNO PHARMCHEM CO.,LTD., we are committed to providing the scientific community with the highest quality Peroxidase, supporting the development of more accurate and efficient diagnostic and research tools.