3-Morpholinopropanesulfonic Acid (MOPS): A Key Biological Buffer for Research and Diagnostics
Essential for stable pH control in biological and biochemical applications.
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3-Morpholinopropanesulfonic acid
As a prominent zwitterionic organic chemical, 3-Morpholinopropanesulfonic acid, commonly known as MOPS, is a highly regarded buffering agent. Introduced as one of Good's buffers in the 1960s, its pKa of 7.20 makes it exceptionally effective for maintaining stable pH levels in biological research and diagnostic applications, crucial for the optimal performance of enzymes and cellular functions. Its utility spans across various scientific disciplines, ensuring reliable experimental outcomes.
- MOPS biological buffer capabilities make it indispensable for precise pH control in sensitive biochemical experiments.
- The stability offered by MOPS pKa 7.20 is critical for maintaining physiological conditions in various biological applications.
- Its application in MOPS buffer for electrophoresis ensures sharp bands and efficient separation of biomolecules.
- When used in MOPS in cell culture, it provides a stable environment supporting cell viability and growth.
Key Advantages of MOPS
Exceptional pH Stability
MOPS offers reliable pH buffering in the critical near-neutral range, vital for preventing drastic pH shifts that could impact biomolecule integrity and reaction kinetics, supporting reproducible scientific findings.
Broad Biological Compatibility
As a key component in MOPS biochemical reagent formulations, it shows low toxicity and minimal interference with biological assays, making it a preferred choice for complex biological systems.
Versatile Application Spectrum
From advanced molecular biology techniques to essential cell culture media and diagnostic kits, MOPS facilitates accurate and efficient processes, contributing to breakthroughs in scientific research and healthcare.
Key Applications
Biological Buffering
The precise buffering capacity of MOPS is essential for maintaining consistent pH in experiments involving enzymes, proteins, and nucleic acids, ensuring accurate results in biochemical studies.
Cell Culture Media
In mammalian cell culture, MOPS helps create a stable pH environment, promoting healthy cell growth and metabolic activity, which is crucial for biotechnology and pharmaceutical research.
Electrophoresis
MOPS is a common buffer component in polyacrylamide gel electrophoresis (PAGE), aiding in the separation and analysis of proteins and nucleic acids by maintaining a consistent pH throughout the process.
Diagnostic Kits
Its reliability makes MOPS an integral part of diagnostic kits, including DNA/RNA extraction and PCR kits, where stable pH is paramount for the accuracy and sensitivity of diagnostic tests.
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