Geneticin (G418 Sulfate): A Powerful Selective Antibiotic for Eukaryotic Cell Culture
Explore the critical role of Geneticin (G418 Sulfate) in cell biology research, from protein synthesis inhibition to enabling geneticin resistance gene selection.
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Geneticin (G418 Sulfate)
Geneticin (G418 Sulfate) is a vital aminoglycoside antibiotic, crucial for selecting eukaryotic cells engineered to express the neomycin resistance gene. Its ability to inhibit protein synthesis makes it indispensable for researchers aiming to maintain transformed cell lines and ensure geneticin resistance gene selection.
- Understanding the geneticin cell culture concentration is key to successful experimental outcomes.
- G418 sulfate serves as a potent selective antibiotic, essential for isolating resistant cell populations.
- Learn how to use geneticin for cell selection effectively by understanding its mechanism.
- The neomycin resistance gene function is central to how Geneticin operates as a selective agent.
Key Advantages
Precise Cell Selection
Leverage Geneticin's precise mechanism to efficiently select for cells harboring specific genetic modifications, ensuring high purity of your cell cultures for reliable research.
Mechanism of Action
Understand the protein synthesis inhibition mechanism of Geneticin, which targets ribosomal subunits to halt polypeptide chain elongation, providing a basis for its selective toxicity.
Research Versatility
Utilize Geneticin for various cell biology applications, including maintaining the integrity of transformed cell lines and ensuring the success of gene transfer experiments.
Key Applications
Eukaryotic Cell Selection
Geneticin is widely employed for the selective cultivation of eukaryotic cells that have been successfully transfected with resistance genes, enabling precise experimental control.
Geneticin Resistance Gene Selection
The compound is critical for identifying and isolating cells that have stably integrated the geneticin resistance gene, a fundamental step in genetic engineering.
Cell Biology Research
Researchers rely on Geneticin for a multitude of cell biology studies, particularly those involving cell line maintenance and the verification of gene expression.
Maintaining Transformed Cell Lines
Ensure the continuous expression and stability of desired genetic modifications by using appropriate geneticin working concentration mammalian cells for maintenance.